A simple and rapid method for isolation of eosinophilic granulocytes from human blood
| Autor: | Pierluigi Patriarca, Pietro Dri, Rita Cramer, Giuliano Zabucchi |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pathology
medicine.medical_specialty Erythrocytes Neutrophils Immunology Cell Separation Biology Granulocyte Toxicology chemistry.chemical_compound Eosinophilic medicine Centrifugation Density Gradient Immunology and Allergy Eosinophilia Humans Centrifugation Physiological values Respiratory Burst Pharmacology Total blood Human blood Povidone Cell Biology respiratory system Eosinophil Isolation (microbiology) Silicon Dioxide Molecular biology Respiratory burst Eosinophils Dextran medicine.anatomical_structure chemistry medicine.symptom Percoll |
| Zdroj: | Journal of leukocyte biology. 52(3) |
| ISSN: | 0741-5400 |
| Popis: | A simple and rapid method for the purification of morphologically and functionally intact eosinophils from human blood of both normal and eosinophilic subjects is described. The method is based on a single centrifugation of total blood leukocytes suspended in Percoll with specific gravity 1.0853 g/ml, after erythrocyte removal by dextran sedimentation. The peculiarity of this isolation technique is the maintenance of strictly physiological values of pH and osmolality throughout the entire procedure. Moreover, the cells are not subjected to measures aimed at changing the physical properties of either neutrophils or eosinophils. Because of such characteristics, this isolation method could be usefully exploited for comparative studies of normal and eosinophilic nor-modense eosinophils and of neutrophils and eosinophils from the same noneosinophilic subject. |
| Databáze: | OpenAIRE |
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