Unequal contribution of two paralogous CENH3 variants in cowpea centromere function
| Autor: | Steven Dreissig, Maximilian Vogt, Joerg Fuchs, Andrew Spriggs, Shamoni Maheshwari, Veit Schubert, Martina Juranić, Dmitri Demidov, Anna M. G. Koltunow, Nial Gursanscky, Tracy How, Rigel Salinas-Gamboa, Takayoshi Ishii, Luca Comai, Andreas Houben, Jean-Philippe Vielle-Calzada, Fernanda de Oliveira Bustamante |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Plant genetics Plant molecular biology QH301-705.5 viruses Centromere Fluorescent Antibody Technique Medicine (miscellaneous) Plant cell biology Biology 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Evolution Molecular 03 medical and health sciences Histone H3 Gene Expression Regulation Plant Genetic variation Gene duplication Biology (General) Gene In Situ Hybridization Fluorescence Phylogeny Centromeres Plant sciences Plant Proteins 030304 developmental biology Genetics Regulation of gene expression 0303 health sciences Vigna fungi Genetic Variation food and beverages Phenotype Organ Specificity Subfunctionalization Ploidy General Agricultural and Biological Sciences Centromere Protein A 010606 plant biology & botany |
| Zdroj: | Communications Biology, 3 Communications Biology Communications Biology, Vol 3, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2399-3642 |
| Popis: | In most diploids the centromere-specific histone H3 (CENH3), the assembly site of active centromeres, is encoded by a single copy gene. Persistance of two CENH3 paralogs in diploids species raises the possibility of subfunctionalization. Here we analysed both CENH3 genes of the diploid dryland crop cowpea. Phylogenetic analysis suggests that gene duplication of CENH3 occurred independently during the speciation of Vigna unguiculata. Both functional CENH3 variants are transcribed, and the corresponding proteins are intermingled in subdomains of different types of centromere sequences in a tissue-specific manner together with the kinetochore protein CENPC. CENH3.2 is removed from the generative cell of mature pollen, while CENH3.1 persists. CRISPR/Cas9-based inactivation of CENH3.1 resulted in delayed vegetative growth and sterility, indicating that this variant is needed for plant development and reproduction. By contrast, CENH3.2 knockout individuals did not show obvious defects during vegetative and reproductive development. Hence, CENH3.2 of cowpea is likely at an early stage of pseudogenization and less likely undergoing subfunctionalization. Communications Biology, 3 ISSN:2399-3642 |
| Databáze: | OpenAIRE |
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