Cestrum strigilatum (Ruiz & Pavón, 1799) B chromosome shares repetitive DNA sequences with A chromosomes of different Cestrum (Linnaeus, 1753) species
| Autor: | Carolina Cristina Quintas, Adriano Alves de Paula, Thiago Fernandes, André Luís Laforga Vanzela, Joana Neres da Cruz Baldissera, Thaíssa Boldieri de Souza |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 rDNA Retrotransposon Plant Science Magnoliopsida 03 medical and health sciences FISH Genus Genetics Plantae Repeated sequence Solanaceae B chromosome biology Cestrum Solanales supernumerary chromosomes Karyotype karyotypes biology.organism_classification microdissection Tracheophyta lcsh:Genetics 030104 developmental biology Fish Animal Science and Zoology Research Article Biotechnology |
| Zdroj: | Comparative Cytogenetics, Vol 11, Iss 3, Pp 511-524 (2017) Comparative Cytogenetics 11(3): 511-524 Comparative Cytogenetics |
| ISSN: | 1993-0771 |
| Popis: | Species of Cestrum (Linnaeus, 1753) have shown large diversity in the accumulation and distribution of repetitive DNA families, and B chromosomes have been described in seven species. Some types of repetitive DNA were identified in A and B chromosomes in species of this plant group, such as AT-rich SSR, 35S and 5S rDNA, C-Giemsa and C-CMA/DAPI bands and retrotransposons. To increase our understanding of the relationships of A and B chromosomes, the B of C. strigilatum Ruiz & Pavón, 1799 was microdissected, amplified and hybridized in situ against chromosomes of this species, and in six other species of this genus. FISH signals were observed in whole the B of C. strigilatum, including stretches of A chromosomes, as well as in some A chromosomes of all tested species. A strong FISH signal was seen adjacent to the 5S rDNA in the proximal region of pair 8 of all species and, due to this, we have searched for 5S rDNA fragments in the microdissected B chromosome. PCR and sequencing data evidenced 5S rDNA deletion along evolutionary pathways of the B of C. strigilatum. Although A and B chromosomes displayed redundancy in the repetitive DNA families in different species, the B of C. strigilatum seemed to differ from those Bs of other Cestrum species by the loss of rDNA fractions. A possible origin of Bs in Cestrum was discussed. |
| Databáze: | OpenAIRE |
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