Scar/WAVE is localised at the tips of protruding lamellipodia in living cells
| Autor: | Penelope Hahne, Stefanie Benesch, J. Victor Small, Antonio Sechi |
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| Rok vydání: | 2001 |
| Předmět: |
animal structures
Biophysics Motility macromolecular substances Biology Transfection Lamellipodium Biochemistry Fluorescence Green fluorescent protein Mice Scar Cell Movement Structural Biology Tumor Cells Cultured Genetics Animals Pseudopodia Wiskott–Aldrich syndrome protein Cytoskeleton Molecular Biology Actin Microfilament Proteins Cell Biology Actins Wiskott-Aldrich Syndrome Protein Family Cell biology Cytoplasm embryonic structures biology.protein biological phenomena cell phenomena and immunity Filopodia |
| Zdroj: | FEBS Letters. 492:215-220 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(01)02239-6 |
| Popis: | Cell motility entails the extension of cytoplasmic processes, termed lamellipodia and filopodia. Extension is driven by actin polymerisation at the tips of these processes via molecular complexes that remain to be characterised. We show here that a green fluorescent protein (GFP) fusion of the Wiskott–Aldrich syndrome protein family member Scar1/WAVE1 is specifically recruited to the tips of lamellipodia in living B16F1 melanoma cells. Scar1–GFP was recruited only to protruding lamellipodia and was absent from filopodia. The localisation of Scar was facilitated by the finding that the formerly described inhibition of lamellipodia formation by ectopical expression of Scar, could be overcome by the treatment of cells with aluminium fluoride. These findings show that Scar is strategically located at sites of actin polymerisation specifically engaged in the protrusion of lamellipodia. |
| Databáze: | OpenAIRE |
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