Effects of silver nanoparticles–polysaccharide on bleomycin-induced pulmonary fibrosis in rats
| Autor: | Mohsen S. Asker, Amer Samir, Manal G. Mahmoud, Amal I. Hassan, Hanan F. Youssef, Sahar S. Mohamed |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Silver Pulmonary Fibrosis Metal Nanoparticles Pharmaceutical Science Apoptosis Nanoconjugates Pharmacology Bleomycin Antioxidants Rats Sprague-Dawley Transforming Growth Factor beta1 Masson's trichrome stain chemistry.chemical_compound Polysaccharides Fibrosis Pulmonary fibrosis medicine Animals Trichrome stain Alcaligenes Respiratory system Lung bcl-2-Associated X Protein Inflammation Antibiotics Antineoplastic Chemistry Monocyte Polysaccharides Bacterial Pneumonia medicine.disease Matrix Metalloproteinases medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Nanoparticles Collagen |
| Zdroj: | Journal of Pharmacy and Pharmacology. 73:1503-1512 |
| ISSN: | 2042-7158 0022-3573 |
| DOI: | 10.1093/jpp/rgab037 |
| Popis: | Objectives The first goal of this study was to synthesize the silver nanoparticles Alcaligenes xylosoxidans exopolysaccharide (Ag-AXEPS). The second objective was to analyse the role of Ag-AXEPS nanoparticles (NPS) in treating bleomycin (BLM)-induced lung fibrosis. Methods Intratracheal bleomycin (2.5 U/kg) was administered to prompt pulmonary fibrosis in rats, and pulmonary fibrosis was treated with Ag-AXEPS nanoparticles (100 ppm/twice a week for four weeks). Key findings Ag-AXEPS nanoparticles significantly decreased the diversity of pulmonary inflammatory agents in rats with BLM-induced fibrosis. Reduced levels of respiratory tumor necrosis factor-alpha, monocyte chemotactic protein-1, matrix metalloproteinases (MMP-2 and MMP-9) were observed on treatment with synthesized Ag-AXEPS. Similarly, the treatment decreased IL-12, mRNA levels of BAX and plasma fibrosis markers like N-terminal procollagen III propeptide and transforming growth factor-β1. On the other hand, the treatment increased mRNA BCL2 and total antioxidant capacity. It also lowered the level of fibrosis, as was shown by a quantified pathologic study of hematoxylin–eosin-stained lung parts. The treatment, however, ensured that lung collagen was restored, as assessed by Masson’s trichrome stain, and that overall survival was increased and enhanced. Conclusions Our work showed that nanoparticles could be obtained at 37°C and may be a possible pulmonary fibrosis therapeutic agent. |
| Databáze: | OpenAIRE |
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