A Single-Cell RNA Expression Map of Human Coronavirus Entry Factors
| Autor: | Cédric Feschotte, Manvendra K. Singh, Vikas Bansal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
viruses Cell Enteroendocrine cell genetics [Peptidyl-Dipeptidase A] metabolism [Nasal Mucosa] genetics [Serine Endopeptidases] medicine.disease_cause Macaque Transcriptome Kidney Tubules Proximal Basal (phylogenetics) 0302 clinical medicine Single-cell analysis Chlorocebus aethiops metabolism [Kidney Tubules Proximal] Receptor lcsh:QH301-705.5 Coronavirus Serine Endopeptidases virus diseases cytology [Kidney Tubules Proximal] virology [Nasal Mucosa] Cell biology medicine.anatomical_structure Receptors Virus Goblet Cells Angiotensin-Converting Enzyme 2 Single-Cell Analysis Coronavirus Infections growth & development [Betacoronavirus] Resource coronaviruses Pneumonia Viral genetics [Viral Tropism] viral receptors ACE2 protein human genetics [Receptors Virus] Peptidyl-Dipeptidase A Biology Article General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences Betacoronavirus Viral entry biology.animal metabolism [Serine Endopeptidases] scRNA-seq medicine Animals Humans ddc:610 metabolism [Peptidyl-Dipeptidase A] Permissive Pandemics Vero Cells Tropism SARS-CoV-2 Gene Expression Profiling HEK 293 cells pathology [Pneumonia Viral] COVID-19 Virus Internalization pathology [Coronavirus Infections] Embryonic stem cell restriction factors Gene expression profiling Nasal Mucosa Viral Tropism 030104 developmental biology Enterocytes HEK293 Cells lcsh:Biology (General) A549 Cells TMPRSS2 protein human Immunology metabolism [Enterocytes] 030217 neurology & neurosurgery metabolism [Goblet Cells] |
| Zdroj: | Cell Reports Cell Reports, Vol 32, Iss 12, Pp 108175-(2020) Cell reports 32(12), 108175 (2020). doi:10.1016/j.celrep.2020.108175 SSRN bioRxiv article-version (status) pre article-version (number) 2 |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2020.108175 |
| Popis: | To predict the tropism of human coronaviruses, we profile 28 SARS-CoV-2 and coronavirus-associated receptors and factors (SCARFs) using single-cell transcriptomics across various healthy human tissues. SCARFs include cellular factors both facilitating and restricting viral entry. Intestinal goblet cells, enterocytes, and kidney proximal tubule cells appear highly permissive to SARS-CoV-2, consistent with clinical data. Our analysis also predicts non-canonical entry paths for lung and brain infections. Spermatogonial cells and prostate endocrine cells also appear to be permissive to SARS-CoV-2 infection, suggesting male-specific vulnerabilities. Both pro- and anti-viral factors are highly expressed within the nasal epithelium, with potential age-dependent variation, predicting an important battleground for coronavirus infection. Our analysis also suggests that early embryonic and placental development are at moderate risk of infection. Lastly, SCARF expression appears broadly conserved across a subset of primate organs examined. Our study establishes a resource for investigations of coronavirus biology and pathology. Graphical Abstract Highlights • Single-cell transcriptome profiling of SCARFs in various somatic and reproductive tissues • Intestine, kidneys, placenta, and spermatogonia appear most permissive for coronavirus • Nasal epithelium exhibits high expression of both promoting and restricting factors • SCARF expression is conserved across the major organs of human, chimpanzee, and macaque Singh et al. provide a resource to predict the tropism and identify the plausible entry points for SARS-CoV-2 and other pathogenic coronaviruses throughout the human body. The RNA levels of 28 genes dubbed “SCARFs,” for SARS-CoV-2 and coronavirus-associated receptors and factors, are profiled in human tissues at the single-cell level. |
| Databáze: | OpenAIRE |
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