Transplantation of neurospheres after granule cell lesions in rats: cognitive improvements despite no long-term immunodetection of grafted cells

Autor: Hélène, Jeltsch, Jason, Yee, Elisabeth, Aloy, Patricia, Marques Pereira, Sarah, Schimchowitsch, Luc, Grandbarbe, Sophie, Caillard, Eliane, Mohier, Jean-Christophe, Cassel, Jeltsch, Hélène, Yee, Jason, Aloy, Elisabeth, Marques Pereira, Patricia, Schimchowitsch, Sarah, Grandbarbe, Luc, Caillard, Sophie, Mohier, Eliane, Cassel, Jean-Christophe
Přispěvatelé: Laboratoire d'Imagerie et de Neurosciences Cognitives (LINC), Université Louis Pasteur - Strasbourg I-IFR37-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2003
Předmět:
Male
Telencephalon
medicine.medical_specialty
Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]
[SDV]Life Sciences [q-bio]
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Morris water navigation task
Water maze
Hippocampal formation
03 medical and health sciences
Behavioral Neuroscience
Mice
0302 clinical medicine
Fetal Tissue Transplantation
Memory
Internal medicine
Neurosphere
medicine
Animals
Brain Tissue Transplantation
Rats
Long-Evans
Maze Learning
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Neurons
0303 health sciences
Analysis of Variance
Dentate gyrus
Graft Survival
Granule cell
Immunohistochemistry
Neural stem cell
Rats
Transplantation
medicine.anatomical_structure
Endocrinology
Dentate Gyrus
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Psychology
Neuroscience
030217 neurology & neurosurgery
Follow-Up Studies
Stem Cell Transplantation
Zdroj: Behavioural Brain Research
Behavioural Brain Research, Elsevier, 2003, 143 (2), pp.177-191. ⟨10.1016/s0166-4328(03)00032-9⟩
ISSN: 0166-4328
1872-7549
DOI: 10.1016/s0166-4328(03)00032-9⟩
Popis: EGF-responsive C17 murine-derived neural stem cells (neurospheres) were grafted into the dentate gyrus of adult male rats after dentate granule cells lesions produced by colchicine injections. Behavioural performance was evaluated over two post-grafting periods, using tests sensitive to hippocampal dysfunctions. The first period began 1 month after grafting and testing conducted in the water maze and the radial maze distinguished working- and reference-memory performance. The second period began 9 months after grafting and learning performance was also evaluated in a Hebb-Williams maze, in addition to both other tests. The lesions induced lasting deficits in all tests. During the first period, the grafts had no effect in either test. Conversely, during the second period, grafted rats showed a weak improvement in the water maze and a significant increase of reference memory performance in the radial maze. In the Hebb-Williams maze, performance of grafted rats was close to normal. Strengthening the idea that dentate gyrus granule cells play an important role in the acquisition of new (perhaps more configural than only spatial) information, our results, moreover, suggest that neurosphere grafts may foster recovery after damage to point-to-point connection systems in the adult brain.
Databáze: OpenAIRE
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