Genetic and flow cytometry analysis of seronegative celiac disease: a cohort study

Autor: Enrique Rodríguez de Santiago, Laura Crespo Pérez, Garbiñe Roy Ariño, Ana De Andrés Martín, Raquel Ríos León, Carlota García Hoz Jiménez, Agustín Albillos, Eugenia Sánchez Rodríguez, Ana Saiz González, Francisco León Prieto
Rok vydání: 2019
Předmět:
Zdroj: Scandinavian Journal of Gastroenterology. 54:563-570
ISSN: 1502-7708
0036-5521
DOI: 10.1080/00365521.2019.1608466
Popis: Background: Seronegative celiac disease (CD) poses a diagnostic challenge.Aims: Characterize and identify differences between seronegative and seropositive CD.Patients and methods: Retrospective cohort study examining adult patients diagnosed with CD (1980–2017). Clinical, analytical, histological, genetic and immunophenotypic data were compiled. Seronegative CD was defined as a anti-tissue transglutaminase type 2 IgA and endomysial antibodies (EMA) negative and HLA-DQ2 and/or DQ8 positive, showing clinical signs of CD plus an abnormal duodenal biopsy, and responding to a gluten-free diet (GFD). Factors associated with seronegative CD were identified through binomial logistic regression.Results: Of 315 CD patients, 289 were seropositive (91.7%) and 26 seronegative (8.3%). Among the seronegative patients, higher prevalence was observed for autoimmune thyroiditis (26.9% vs. 9.7%, p = .016), HLA-DQ8 heterozygosity (23.1% vs. 2.5%, p ˂ .001) and Marsh I lesion (34.6% vs. 3.7%, p ˂ .001). The two group...
Databáze: OpenAIRE