Discovery and characterisation of an amidine-containing ribosomally-synthesised peptide that is widely distributed in nature
| Autor: | Andrew W. Truman, Natalia M. Vior, Edward S. Hems, Rodney Lacret, Alicia H Russell |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Natural product biology 010405 organic chemistry Firmicutes Peptide General Chemistry Computational biology 010402 general chemistry biology.organism_classification 01 natural sciences 0104 chemical sciences Actinobacteria Amidine 03 medical and health sciences chemistry.chemical_compound Chemistry chemistry Streptomyces albidoflavus Genome mining Gene Streptomyces albus 030304 developmental biology |
| Zdroj: | Chemical Science |
| ISSN: | 2041-6520 |
| Popis: | Ribosomally synthesised and post-translationally modified peptides (RiPPs) are a structurally diverse class of natural product with a wide range of bioactivities. Genome mining for RiPP biosynthetic gene clusters (BGCs) is often hampered by poor annotation of the short precursor peptides that are ultimately modified into the final molecule. Here, we utilise a previously described genome mining tool, RiPPER, to identify novel RiPP precursor peptides near YcaO-domain proteins, enzymes that catalyse various RiPP post-translational modifications including heterocyclisation and thioamidation. Using this dataset, we identified a novel and diverse family of RiPP BGCs spanning over 230 species of Actinobacteria and Firmicutes. A representative BGC from Streptomyces albidoflavus J1074 (formerly known as Streptomyces albus) was characterised, leading to the discovery of streptamidine, a novel amidine-containing RiPP. This new BGC family highlights the breadth of unexplored natural products with structurally rare features, even in model organisms. Genome mining for pathways containing YcaO proteins revealed a widespread novel family of RiPP gene clusters. A model gene cluster was characterised through genetic and chemical analyses, which yielded streptamidine, a novel amidine-containing RiPP. |
| Databáze: | OpenAIRE |
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