Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
| Autor: | Gabriel Lucca de Oliveira Salvador, Flavio Queiroz-Telles, Rosangela Lameira Pinheiro, Patricia F. Herkert, Arnaldo Lopes Colombo, Jacques F. Meis, Renata R. Gomes, Carla Sakuma de Oliveira, Marcelo Simão Ferreira, Marisol Dominguez Muro, Vania A. Vicente, Ferry Hagen, Alexandre Vargas Schwarzbold |
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| Přispěvatelé: | Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Medical Mycology |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Antifungal Agents Cryptococcosis/microbiology Genotype 030106 microbiology Population Drug Resistance Cryptococcus Microbial Sensitivity Tests Microbiology 03 medical and health sciences Drug Resistance Fungal parasitic diseases medicine Humans Polymorphism Amplified Fragment Length Polymorphism Analysis education Phylogeny Cryptococcus neoformans education.field_of_study biology Cryptococcosis General Medicine biology.organism_classification medicine.disease bacterial infections and mycoses Cryptococcus neoformans/classification lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4] Fungal Restriction Fragment Length Antifungal Agents/pharmacology Microsatellite Amplified fragment length polymorphism Restriction fragment length polymorphism Brazil Polymorphism Restriction Fragment Length Multilocus Sequence Typing |
| Zdroj: | Journal of Medical Microbiology, 67(4), 560-569. Society for General Microbiology Journal of Medical Microbiology, 67, 560-569 Journal of Medical Microbiology, 67, 4, pp. 560-569 |
| ISSN: | 0022-2615 |
| Popis: | Contains fulltext : 190959.pdf (Publisher’s version ) (Closed access) PURPOSE: Cryptococcosis is acquired from the environment by the inhalation of Cryptococcus cells and may establish from an asymptomatic latent infection into pneumonia or meningoencephalitis. The genetic diversity of a Cryptococcus neoformans species complex has been investigated by several molecular tools, such as multi-locus sequence typing, amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism and microsatellite analysis. This study aimed to investigate the genotype distributions and antifungal susceptibility profiles of C. neoformans sensu lato isolates from southern Brazil. METHODOLOGY: We studied 219 C. neoformans sensu lato isolates with mating- and serotyping, AFLP fingerprinting, microsatellite typing and antifungal susceptibility testing.Results/Key findings. Among the isolates, 136 (69 %) were from HIV-positive patients. Only C. neoformans mating-type alpha and serotype A were observed. AFLP fingerprinting analysis divided the isolates into AFLP1/VNI (n=172; 78.5 %), AFLP1A/VNII (n=19; 8.7 %), AFLP1B/VNII (n=4; 1.8 %) and a new AFLP pattern AFLP1C (n=23; 10.5 %). All isolates were susceptible to tested antifungals and no correlation between antifungal susceptibility and genotypes was observed. Through microsatellite analysis, most isolates clustered in a major microsatellite complex and Simpson's diversity index of this population was D=0.9856. CONCLUSION: The majority of C. neoformans sensu stricto infections occurred in HIV-positive patients. C. neoformans AFLP1/VNI was the most frequent genotype and all antifungal drugs had high in vitro activity against this species. Microsatellite analyses showed a high genetic diversity within the regional C. neoformans sensu stricto population, and correlation between environmental and clinical isolates, as well as a temporal and geographic relationship. |
| Databáze: | OpenAIRE |
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