Predesquamin inhibits desquamation
| Autor: | Trace Bell, Chana H Santschi, Miriam M. Brysk, Srinivasan Rajaraman |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Ethylenediaminetetraacetic acid
In Vitro Techniques Desquamation Receptors Concanavalin A chemistry.chemical_compound Lectins Endopeptidases medicine Stratum corneum Humans Chelation Bony Callus Protein Precursors Glycoproteins chemistry.chemical_classification integumentary system biology Epidermis (botany) Cell Differentiation Cell Biology Molecular biology medicine.anatomical_structure chemistry Biochemistry Epidermal Cells Polyclonal antibodies Callus biology.protein medicine.symptom Epidermis Glycoprotein Cell Adhesion Molecules |
| Zdroj: | Experimental cell research. 209(2) |
| ISSN: | 0014-4827 |
| Popis: | Predesquamin is a Con A binding glycoprotein from human epidermis, with a molecular weight of 650 kDa. Its isolation and characterization are detailed, as well as the preparation of a polyclonal antibody to it. The antibody labels primarily the lower stratum corneum in fluorescent and ultrastructural immunolocalization, both in normal skin and plantar callus. A desquamation model system, in which plantar callus is incubated with the chelating agent ethylenediaminetetraacetic acid (EDTA) is refined by substituting for EDTA the Ca 2+ -specific ethylene glycol bis(β-aminoethyl ether)- N,N′ -tetraacetic acid. Moreover, the shedding activity can be suppressed by raising the Ca 2+ concentration. Within this model, it is demonstrated that predesquamin at nanomolar concentrations inhibits squame shedding in a dose-dependent manner. We conclude that predesquamin plays an important role in blocking premature desquamation of the lower stratum corneum. |
| Databáze: | OpenAIRE |
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