Optofluidic detection of Zika nucleic acid and protein biomarkers using multimode interference multiplexing
| Autor: | Alexandra Stambaugh, Joshua W. Parks, M. A. Stott, Holger Schmidt, Aaron R. Hawkins, G. G. Meena |
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| Rok vydání: | 2018 |
| Předmět: |
biology
Protein biomarkers 02 engineering and technology Computational biology 021001 nanoscience & nanotechnology biology.organism_classification Multiplexing Article Atomic and Molecular Physics and Optics Zika virus Biomarker (cell) 020210 optoelectronics & photonics 0202 electrical engineering electronic engineering information engineering Nucleic acid Multiplex Multimode interference 0210 nano-technology Biotechnology Antibody detection |
| Zdroj: | Biomedical Optics Express. 9:3725 |
| ISSN: | 2156-7085 |
| Popis: | The recent massive Zika virus (ZIKV) outbreak illustrates the need for rapid and specific diagnostic techniques. Detecting ZIKV in biological samples poses unique problems: antibody detection of ZIKV is insufficient due to cross-reactivity of Zika antibodies with other flaviviruses, and nucleic acid and protein biomarkers for ZIKV are detectable at different stages of infection. Here, we describe a new optofluidic approach for the parallel detection of different molecular biomarkers using multimode interference (MMI) waveguides. We report differentiated, multiplex detection of both ZIKV biomarker types using multi-spot excitation at two visible wavelengths with over 98% fidelity by combining several analysis techniques. |
| Databáze: | OpenAIRE |
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