Comparison of Programmed Death Ligand-1 Immunohistochemical Staining Between Endobronchial Ultrasound Transbronchial Needle Aspiration and Resected Lung Cancer Specimens
| Autor: | John J. Mullon, Dante N. Schiavo, James R. Jett, Marie Christine Aubry, Darlene R. Nelson, David E. Midthun, Kenneth K. Sakata, Eric S. Edell, Ryan Kern |
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| Rok vydání: | 2018 |
| Předmět: |
Pulmonary and Respiratory Medicine
Adult Male Lung Neoplasms Concordance Adenocarcinoma Critical Care and Intensive Care Medicine Stain B7-H1 Antigen Endosonography 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Biopsy Bronchoscopy medicine Carcinoma Biomarkers Tumor Humans Lung cancer Endoscopic Ultrasound-Guided Fine Needle Aspiration Aged Retrospective Studies Aged 80 and over medicine.diagnostic_test business.industry Cancer Retrospective cohort study Middle Aged medicine.disease Immunohistochemistry 030228 respiratory system 030220 oncology & carcinogenesis Carcinoma Squamous Cell Carcinoma Large Cell Female Cardiology and Cardiovascular Medicine Nuclear medicine business |
| Zdroj: | Chest. 154(4) |
| ISSN: | 1931-3543 |
| Popis: | Background In advanced non-small cell lung cancer (NSCLC), small biopsy specimens from endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) are often the only available material from cancer tissue for the analysis of programmed death ligand-1 (PD-L1) expression. We aim to assess the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of PD-L1 expression at ≥ 1% and ≥ 50% on EBUS-TBNA samples compared with their corresponding surgically resected tumor. Methods We retrospectively reviewed all patients who underwent EBUS-TBNA followed by surgical resection of NSCLC between July 2006 and September 2016. Demographic information and periprocedural/surgical data were collected. The archived specimens were retrieved and assessed for PD-L1. A positive PD-L1 stain was defined using two separate cutoff points: ≥ 1% and ≥ 50% of tumor cell positivity. EBUS-TBNA aspirates were compared with the surgically resected specimen to calculate the sensitivity, specificity, PPV, and NPV. Results Sixty-one patients were included. For PD-L1 ≥ 1%, the sensitivity, specificity, PPV, and NPV were 72%, 100%, 100%, and 80%, respectively. For PD-L1 ≥ 50%, the sensitivity, specificity, PPV, and NPV were 47%, 93%, 70%, and 84%, respectively. The concordance rates for PD-L1 ≥ 1% and ≥ 50% were 87% and 82%, respectively. Conclusions A PD-L1 cutoff of ≥ 1% on EBUS-TBNA has a strong correlation with resected tumor specimen. For PD-L1 ≥ 50%, there is a significant decrease in the sensitivity and PPV of EBUS-TBNA specimen when compared with resected tumor. When analyzing for PD-L1 expression using a cutoff of ≥ 50%, EBUS-TBNA specimens may misclassify the status of PD-L1. |
| Databáze: | OpenAIRE |
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