Effect of fasting on PPARγ and AMPK activity in adipocytes
| Autor: | Takahide Ikeda, Mitsuru Seishima, Hiroyuki Morita, Yoshihiro Uno, Tomoatsu Mune, Chiyo Sugiyama, Jun Takeda, Tatsuo Ishizuka, Masami Matsumoto, Kenji Matsubara, Masao Takemura, Kazuo Kajita |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Adipose tissue chemistry.chemical_compound Endocrinology Internal medicine Adipocyte Gene expression Adipocytes Internal Medicine medicine Animals Rats Wistar Protein kinase A Regulation of gene expression Lipoprotein lipase business.industry Adenylate Kinase AMPK Fasting General Medicine Ribonucleotides Aminoimidazole Carboxamide Rats PPAR gamma Adipose Tissue chemistry Energy Metabolism business Intracellular |
| Zdroj: | Diabetes Research and Clinical Practice. 81:144-149 |
| ISSN: | 0168-8227 |
| Popis: | We investigated the effects of fasting on gene expression and intracellular signals regulating energy metabolism in adipose tissue. Following fasting for 15h or 39h, epididymal fat pads were isolated from Wistar rats. PPARgamma mRNA levels decreased in the adipose tissues isolated from rats fasted for 39h, whereas adipocyte lipid-binding protein (aP2) and lipoprotein lipase (LPL) mRNA levels increased. Overnight fasting increased the AMP/ATP ratio and AMP-activated protein kinase (AMPK) in adipose tissue, but not in muscle or liver tissue. In addition, the effect of 5-aminoimidazole-4-carboxyamide-ribonucleoside (AICAR) on PPARgamma expression in primary cultured adipocytes was investigated. AICAR reduced PPARgamma mRNA levels but increased aP2 and LPL mRNA levels. Thus, fasting-induced AMPK activation may affect on the regulation of gene expression in adipocytes. |
| Databáze: | OpenAIRE |
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