Simultaneous Determination of Cortisol, Cortisone, and Multiple Illicit Drugs in Hair among Female Drug Addicts with LC-MS/MS
| Autor: | Shenghuo Chen, Jin Yang, Yan Wu, Yun Pu, Huihua Deng, Cailing Duan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Hydrocortisone
illicit drugs Pharmaceutical Science hair matrix Pharmacology cortisol 01 natural sciences Article Analytical Chemistry Heroin lcsh:QD241-441 03 medical and health sciences Drug withdrawal 0302 clinical medicine lcsh:Organic chemistry Limit of Detection Tandem Mass Spectrometry Drug Discovery mental disorders medicine Humans Ketamine Physical and Theoretical Chemistry LC-MS/MS business.industry 010401 analytical chemistry Organic Chemistry cortisone MDMA Methamphetamine medicine.disease 0104 chemical sciences Chemistry (miscellaneous) Drug addict Morphine Molecular Medicine Female Cortisone business 030217 neurology & neurosurgery medicine.drug Chromatography Liquid Hair |
| Zdroj: | Molecules, Vol 26, Iss 516, p 516 (2021) Molecules Volume 26 Issue 2 |
| ISSN: | 1420-3049 |
| Popis: | Long-term dependence of illicit drugs impairs the function of the hypothalamic-pituitary-adrenal (HPA) axis, which regulates the secretion of endogenous steroids, cortisol, and cortisone. Thus, the present study aimed to develop a sensitive method for simultaneous determination of the multiple illicit drugs and two steroids in hair to monitor the status of illicit drug exposure and the physiological and psychological health of drug addicts. The target analytes were extracted from hair by incubation with 1 mL methanol for 24 h at 40 ° C and then determined with LC-APCI+-MS/MS. The validated method showed acceptable linearity (R2 > 0.99) in the range of 1.25&ndash 250 pg/mg for cortisol and cortisone, 2.5&ndash 125 pg/mg for heroin, 2.5&ndash 1250 pg/mg for ketamine, 2.5&ndash 5000 pg/mg for methamphetamine (MAM), 2.5&ndash 250 pg/mg for 3, 4-methylenedioxymethamphetamine (MDMA), morphine, and 6-monoacetylmorphine (6-AM). Limits of quantification were 1.6, 1.2, 1.6, 1.0, 1.4, 0.3, 2.1, and 1.2 pg/mg for cortisol, cortisone, heroin, ketamine, MAM, MDMA, morphine, and 6-AM, respectively. Method recoveries were from 90&ndash 115% for all analytes. Inter-day and intra-day coefficients of variation were within 10%. Finally, this method was successfully applied to detect the aforementioned analytes in hair among female drug addicts who self-reported to be MAM abuser, heroin abuser, ketamine abuser, and abuser of mixture drugs of MAM and heroin. MAM abusers with current MAM use showed significantly higher concentrations of cortisol, MAM, and MDMA than controls with drug withdrawal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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