Quantitative analysis of erythromycin by reversed-phase liquid chromatography using column-switching
| Autor: | Jos Hoogmartens, K De Turck, T Cachet, Eugene Roets |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Acetonitriles
Chromatography Chemistry Clinical Biochemistry Temperature Analytical chemistry Pharmaceutical Science Erythromycin Reversed-phase chromatography Buffers Dosage form Analytical Chemistry Volumetric flow rate Quaternary Ammonium Compounds Impurity Phase (matter) Calibration Drug Discovery medicine Column switching Quantitative analysis (chemistry) Chromatography High Pressure Liquid Spectroscopy medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 9:547-555 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/0731-7085(91)80176-a |
| Popis: | A column-switching technique is described for LC of erythromycin. The method allows, in about 1 h, the separation of erythromycin A from all its known potential impurities, except erythromycin D, which is a minor impurity. The switching technique combines two columns (7.5 cm x 4.6 mm and 25.0 cm x 4.6 mm) both packed with RSil C 18 LL 10 microns. The mobile phase is acetonitrile-tetrabutylammonium sulphate (0.2 M, pH 6.0)-ammonium phosphate buffer (0.2 M, pH 6.0)-water (24:5:5:66, v/v/v/v). Temperature was 35 degrees C, flow rate was 1.5 ml min-1, detection was by UV at 210 nm. Results for a number of commercial samples of various origin are reported. |
| Databáze: | OpenAIRE |
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