Does longer storage of blastocysts with equal grades in a cryopreserved state affect the perinatal outcomes?
| Autor: | Chen Huanhua, Hong Zhou, Xianyou Gan, Jinhui Shu, Kongrong Xu, Caizhu Wang, Ruoyun Lin, Xin Zhao |
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| Rok vydání: | 2021 |
| Předmět: |
Pregnancy Rate
Affect (psychology) General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology Embryo Culture Techniques Pregnancy medicine Humans Blastocyst Survival rate reproductive and urinary physiology Aged Retrospective Studies urogenital system business.industry Infant Newborn General Medicine Vitrification Abortion rate Young age medicine.anatomical_structure Neonatal outcomes embryonic structures Female General Agricultural and Biological Sciences Live birth business |
| Zdroj: | Cryobiology. 103 |
| ISSN: | 1090-2392 |
| Popis: | Aim Although mammalian embryos could be preserved in liquid nitrogen for thousands of years in theoretical models, the viability of cryopreserved blastocyst with varying grades remains to be speculated. In this study, we aimed to determine whether the longer storage time of blastocysts with equal grades could negatively affect the perinatal outcomes. Materials and methods Single vitrified-warmed blastocyst was divided into four grades (AA, AB/BA, BB, BC/CB) according to the blastocyst score when freezing, and each grade of blastocyst was categorized into four storage duration categories: 28 days-1 year, 1–3 years, 3–5 years, and ≥5 years. Then the perinatal outcomes with different storage time were analyzed. Results Our results revealed that for blastocysts with the same grade, the length of storage time had no statistical effect on blastocyst survival rate, clinical pregnancy/implantation rate, live birth rate, and abortion rate. In addition, more advanced developmental blastocyst could obtain better pregnancy outcomes regardless of the cryopreservation length. Similar neonatal outcomes were obtained over time. Conclusions Cryopreservation time could not negatively affect the perinatal outcomes of blastocysts with equal grades. Efficient blastocyst cryopreservation technology by vitrification can help older women obtain high-quality embryos at a young age. |
| Databáze: | OpenAIRE |
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