Arabidopsis thaliana sucrose phosphate synthase ( sps ) genes are expressed differentially in organs and tissues, and their transcription is regulated by osmotic stress
| Autor: | Miguel Martínez-Trujillo, Joel E. López-Meza, José López-Bucio, León Francisco Ruiz-Herrera, Yazmín Carreón-Abud, María Gloria Solís-Guzmán, Lenin Sánchez-Calderón, Gerardo R. Argüello-Astorga |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Osmotic shock education Arabidopsis Plant Roots 01 natural sciences Gene Expression Regulation Enzymologic 03 medical and health sciences Gene Expression Regulation Plant Genes Reporter Osmotic Pressure Genetics Tissue Distribution Promoter Regions Genetic Molecular Biology Gene chemistry.chemical_classification Reporter gene ATP synthase biology Arabidopsis Proteins Gene Expression Profiling fungi Gene Expression Regulation Developmental Promoter biology.organism_classification 030104 developmental biology Enzyme chemistry Biochemistry Glucosyltransferases Multigene Family biology.protein Sucrose-phosphate synthase 010606 plant biology & botany Developmental Biology |
| Zdroj: | Gene Expression Patterns. :92-101 |
| ISSN: | 1567-133X |
| Popis: | Sucrose is synthesized from UDP-Glc and Fru-6-phosphate via the activity of sucrose-phosphate synthase (SPS) enzymes, which produce Suc-6-phosphate. Suc-6-phosphate is rapidly dephosphorylated by phosphatases to produce Suc and inorganic phosphate. Arabidopsis has four sps genes encoding SPS enzymes. Of these enzymes, AtSPS1F and AtSPS2F have been grouped with other dicotyledonous SPS enzymes, while AtSPS3F and AtSPS4F are included in groups with both dicotyledonous and monocotyledonous SPS enzymes. In this work, we generated Arabidopsis thaliana transformants containing the promoter region of each sps gene fused to gfp::uidA reporter genes. A detailed characterization of expression conferred by the sps promoters in organs and tissues was performed. We observed expression of AtSPS1F, AtSPS2F and AtSPS3F in the columella roots of the plants that support sucrose synthesis. Hence, these findings support the idea that sucrose synthesis occurs in the columella cells, and suggests that sucrose has a role in this tissue. In addition, the expression of AtSPS4F was identified in embryos and suggests its participation in this developmental stage. Quantitative transcriptional analysis of A. thaliana plants grown in media with different osmotic potential showed that AtSPS2F and AtSPS4F respond to osmotic stress. |
| Databáze: | OpenAIRE |
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