Antibody to Thrombin Receptor Inhibits Neointimal Smooth Muscle Cell Accumulation Without Causing Inhibition of Platelet Aggregation or Altering Hemostatic Parameters After Angioplasty in Rat
| Autor: | Shinji Yoshitake, Toshie Yamada, Takashi Musha, Mamoru Yanagimachi, Motoji Kogushi, Hiroko Kobayashi, Takanori Kawamura, Osamu Ito, Hiroshi Tanaka, Masashi Ito, Masahiro Takada, Isao Saito, Fusayo Yoshida |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Platelet Aggregation Physiology Thrombin Time Cell Muscle Smooth Vascular Catheterization Rats Sprague-Dawley Immune system Thrombin In vivo Proliferating Cell Nuclear Antigen Internal medicine Thrombin receptor medicine Animals Platelet Cells Cultured Hemostasis biology Cell growth Angioplasty Fibrinogen Rats Carotid Arteries medicine.anatomical_structure Endocrinology Immunology Immunologic Techniques biology.protein Receptors Thrombin Antibody Tunica Intima Cardiology and Cardiovascular Medicine Oligopeptides Cell Division Signal Transduction medicine.drug |
| Zdroj: | Circulation Research. 82:980-987 |
| ISSN: | 1524-4571 0009-7330 |
| Popis: | Abstract —An antibody was raised in rabbits against SFFLRNPSEDTFEQF peptide, which is an NH 2 -terminal peptide of the thrombin-cleaved rat thrombin receptor. In vitro, the antibody inhibited rat smooth muscle cell proliferation but had no effect on rat platelet aggregation or clotting time. These data indicate that the antibody is a specific blocker of the thrombin receptor–signaling pathway in rat smooth muscle cells but does not work as a blocker in rat platelets, suggesting the existence of a second thrombin receptor in the platelets. Using an in vivo balloon catheter–induced injury model in rats, we examined the effect of the anti-rat thrombin receptor IgG on intimal smooth muscle cell accumulation 2 weeks after angioplasty. Analysis of the ratio of intimal to medial cross-sectional areas showed that injection of immune IgG resulted in 43.7% and 53.1% reduction ( P P |
| Databáze: | OpenAIRE |
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