Differential expression of DNA topoisomerase I gene between CPT-11 acquired- and native-resistant human pancreatic tumor cell lines: detected by RNA/PCR-based quantitation assay
| Autor: | Sachi Takeda, Keiki Sato, Takashi Tsuruo, Yoshikazu Sugimoto, Takao Shimazoe, Akira Kono |
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| Rok vydání: | 1992 |
| Předmět: |
Molecular Sequence Data
Biophysics Drug Resistance Gene Expression Irinotecan Biochemistry Polymerase Chain Reaction Gene Expression Regulation Enzymologic Cell Line Gene expression Humans Northern blot RNA Messenger Molecular Biology Gene Messenger RNA biology Base Sequence Topoisomerase Wild type Cell Biology Blotting Northern Molecular biology Antineoplastic Agents Phytogenic Blot Gene Expression Regulation Neoplastic Pancreatic Neoplasms DNA Topoisomerases Type I Oligodeoxyribonucleotides Cell culture biology.protein Camptothecin Cell Division |
| Zdroj: | Biochemical and biophysical research communications. 184(2) |
| ISSN: | 0006-291X |
| Popis: | RNA PCR quantitation method was developed to determine DNA Topoisomerase I(Topo I)-specific mRNA in order to study its gene expression in CPT-11 sensitive, acquired- or native-resistant human pancreatic tumor cell lines. The results were supported by Northern blotting and Western blotting analyses. Acquired-resistant cells have shown decreased levels of Topo I mRNA, compared with their parental cells. On the contrary, in the wild type cells no correlation was shown between sensitivity and gene expression. On the other specific Topo I activity of the native resistant cell lines was fairly lower than that of sensitive cell lines, suggesting that immunoreactive Tope I protein contains low levels of active form enzyme which could be targets of CPT-11 in these native-resistant ones. Finally, the different mechanisms might be operative between acquired- and native-resistant tumor cells. |
| Databáze: | OpenAIRE |
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