Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
| Autor: | Lisheng Peng, Xiuqing Xiao, Tian Zhang, Yashuang Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Mesenchyme Human Embryonic Stem Cells Germ layer Biology Cell Line 03 medical and health sciences 0302 clinical medicine medicine CRISPR Humans Clustered Regularly Interspaced Short Palindromic Repeats Biology (General) Gene Embryonic Stem Cells Cas9 Cell Biology General Medicine Cell biology 030104 developmental biology medicine.anatomical_structure Forebrain Homeobox Female CRISPR-Cas Systems 030217 neurology & neurosurgery Developmental Biology Human embryonic stem cell line |
| Zdroj: | Stem Cell Research, Vol 53, Iss, Pp 102309-(2021) |
| ISSN: | 1876-7753 |
| Popis: | Human ALX1 gene (ALX Homeobox 1) is a protein coding gene and gene ontology annotations related to this gene include DNA-binding transcription factor activity and protein heterdimerization activity. It is necessary for survival of forebrain mesenchyme and may be involved in development of cervix. However, the function of the gene has yet to be determined in humans. Here we generated an ALX1 homozygous human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. The WAe001-A-060 has a normal undifferentiated morphology and karyotype, pluripotency and three germ layers differentiation potential in vivo. |
| Databáze: | OpenAIRE |
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