Soluble plantain fibre blocks adhesion and M-cell translocation of intestinal pathogens

Autor: Jonathan M. Rhodes, Maelle Prorok-Hamon, Craig Winstanley, Carol L. Roberts, Åsa V. Keita, Paul Knight, Niamh O′Kennedy, Bryony N. Parsons, Johan D. Söderholm, Barry J. Campbell
Rok vydání: 2013
Předmět:
Dietary Fiber
Male
Salmonella typhimurium
Enteric infections
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
FAE
follicle-associated epithelium
Chromosomal translocation
medicine.disease_cause
Biochemistry
Bacterial Adhesion
Peyer's Patches
LB
Luria-Bertani
CFU
colony forming units
0302 clinical medicine
M-cell
membranous/microfold cell
ANOVA
analysis of variance
MOI
multiplicity of infection
Aged
80 and over
Colony-forming unit
0303 health sciences
Nutrition and Dietetics
TEER
trans-epithelial electrical resistance
Dietary fibre
TEM
transmission electron microscopy
PP
Peyer′s patches
Adhesion
Middle Aged
PBS
Phosphate-buffered saline
Diarrhoea
M (microfold) cell
Mucosal immunology
Female
030211 gastroenterology & hepatology
Research Article
Shigella sonnei
Biology
Peyer′s patches
03 medical and health sciences
FBS
fetal bovine serum
DMEM
Dulbecco′s modified Eagle′s medium
Escherichia coli
medicine
Humans
Molecular Biology
Aged
030304 developmental biology
Clostridioides difficile
Prebiotic
Musa
NSP
non-starch polysaccharides
Solubility
Caco-2
Bacterial Translocation
Caco-2 Cells
Fetal bovine serum
Zdroj: The Journal of Nutritional Biochemistry
ISSN: 0955-2863
DOI: 10.1016/j.jnutbio.2012.02.013
Popis: Dietary fibres may have prebiotic effects mediated by promotion of beneficial bacteria. This study explores the possibility that soluble plant fibre may also improve health by inhibiting epithelial adhesion and translocation by pathogenic bacteria. We have focussed on soluble non-starch polysaccharide (NSP) from plantain bananas (Musa spp.) which previous studies showed to be particularly effective at blocking Escherichia coli epithelial adherence. In vitro and ex vivo studies assessed the ability of plantain NSP to inhibit epithelial cell adhesion and invasion of various bacterial pathogens, and to inhibit their translocation through microfold (M)-cells and human Peyer's patches mounted in Ussing chambers. Plantain NSP showed dose-related inhibition of epithelial adhesion and M-cell translocation by a range of pathogens. At 5mg/ml, a concentration readily achievable in the gut lumen, plantain NSP inhibited adhesion to Caco2 cells by Salmonella Typhimurium (85.0 ± 8.2%, P.01), Shigella sonnei (46.6 ± 29.3%, P.01), enterotoxigenic E.coli (56.1 ± 23.7%, P.05) and Clostridium difficile (67.6 ± 12.3%, P.001), but did not inhibit adhesion by enteropathogenic E.coli. Plantain NSP also inhibited invasion of Caco2 cells by S. Typhimurium (80.2 ± 9.7%) and Sh. sonnei (46.7 ± 13.4%); P.01. Plantain NSP, 5mg/ml, also inhibited translocation of S. Typhimurium and Sh. sonnei across M-cells by 73.3 ± 5.2% and 46.4 ± 7.7% respectively (P.05). Similarly, S. Typhimurium translocation across Peyer's patches was reduced 65.9 ± 8.1% by plantain NSP (P.01). Soluble plantain fibre can block epithelial adhesion and M-cell translocation of intestinal pathogens. This represents an important novel mechanism by which soluble dietary fibres can promote intestinal health and prevent infective diarrhoea.
Databáze: OpenAIRE
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