Polymorphisms of the FTO and MTHFR genes and vascular, inflammatory and metabolic marker levels in postmenopausal women

Autor: Ar Genazzani, Jasson Espinoza-Caicedo, Tommaso Simoncini, Gustavo S. Escobar, Peter Chedraui, Faustino R. Pérez-López, Maria Magdalena Montt-Guevara
Rok vydání: 2016
Předmět:
0301 basic medicine
Endocrinology
Diabetes and Metabolism
Visfatin
030204 cardiovascular system & hematology
Polymerase Chain Reaction
0302 clinical medicine
Endocrinology
Medicine
FTO
Genotypes
Metabolic syndrome
MTHFR
Polymorphisms
Postmenopause
sCD40L
Adult
Aged
Alpha-Ketoglutarate-Dependent Dioxygenase FTO
Biomarkers
Case-Control Studies
Female
Humans
Inflammation
Metabolic Syndrome
Methylenetetrahydrofolate Reductase (NADPH2)
Middle Aged
Obesity
Polymorphism
Single Nucleotide
Vascular Diseases
biology
Leptin
Single Nucleotide
Diabetes and Metabolism
medicine.medical_specialty
Single-nucleotide polymorphism
03 medical and health sciences
Internal medicine
SNP
Polymorphism
Adiponectin
business.industry
medicine.disease
030104 developmental biology
Methylenetetrahydrofolate reductase
biology.protein
Resistin
business
Zdroj: Journal of Endocrinological Investigation. 39:885-890
ISSN: 1720-8386
Popis: To determine the prevalence of three single nucleotide polymorphisms (SNPs) in postmenopausal women with and without the metabolic syndrome (METS) and to explore levels of circulating biomarkers of inflammation, vascular and metabolic dysfunction according to SNP genotypes. DNA was extracted from the whole blood of 192 natural postmenopausal women (40 to 65 years) screened for the METS and tested for three gene SNPs related to obesity: the fat mass obesity (FTO: rs9939609) and the methylenetetrahydrofolate reductase (MTHFR: C677T and A1298C). Blood levels of angiopoietin, IL-8, sFASL, IL-6, TNF-α, sCD40L, PAI-1, u-PA, leptin, adiponectin, resistin, ghrelin, visfatin, adipsin and insulin were measured in a subgroup, with and without the METS, using multiplex technology (n = 100) and compared according to SNP genotypes. Genotype frequency of the three studied SNPs did not differ in relation to the presence of the METS. However, genotypes CT+TT (C677T) and AT (rs9939609) were more prevalent in women with high triglyceride levels. Pooled sub-analysis (n = 100) found that median sCD40L and visfatin levels were higher in women with genotypes AT+TT (rs9939609) as compared to AA (1178 vs. 937.0 pg/mL and 0.93 vs. 0.43 ng/mL, respectively, p
Databáze: OpenAIRE
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