Polymorphisms of the FTO and MTHFR genes and vascular, inflammatory and metabolic marker levels in postmenopausal women
Autor: | Ar Genazzani, Jasson Espinoza-Caicedo, Tommaso Simoncini, Gustavo S. Escobar, Peter Chedraui, Faustino R. Pérez-López, Maria Magdalena Montt-Guevara |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism Visfatin 030204 cardiovascular system & hematology Polymerase Chain Reaction 0302 clinical medicine Endocrinology Medicine FTO Genotypes Metabolic syndrome MTHFR Polymorphisms Postmenopause sCD40L Adult Aged Alpha-Ketoglutarate-Dependent Dioxygenase FTO Biomarkers Case-Control Studies Female Humans Inflammation Metabolic Syndrome Methylenetetrahydrofolate Reductase (NADPH2) Middle Aged Obesity Polymorphism Single Nucleotide Vascular Diseases biology Leptin Single Nucleotide Diabetes and Metabolism medicine.medical_specialty Single-nucleotide polymorphism 03 medical and health sciences Internal medicine SNP Polymorphism Adiponectin business.industry medicine.disease 030104 developmental biology Methylenetetrahydrofolate reductase biology.protein Resistin business |
Zdroj: | Journal of Endocrinological Investigation. 39:885-890 |
ISSN: | 1720-8386 |
Popis: | To determine the prevalence of three single nucleotide polymorphisms (SNPs) in postmenopausal women with and without the metabolic syndrome (METS) and to explore levels of circulating biomarkers of inflammation, vascular and metabolic dysfunction according to SNP genotypes. DNA was extracted from the whole blood of 192 natural postmenopausal women (40 to 65 years) screened for the METS and tested for three gene SNPs related to obesity: the fat mass obesity (FTO: rs9939609) and the methylenetetrahydrofolate reductase (MTHFR: C677T and A1298C). Blood levels of angiopoietin, IL-8, sFASL, IL-6, TNF-α, sCD40L, PAI-1, u-PA, leptin, adiponectin, resistin, ghrelin, visfatin, adipsin and insulin were measured in a subgroup, with and without the METS, using multiplex technology (n = 100) and compared according to SNP genotypes. Genotype frequency of the three studied SNPs did not differ in relation to the presence of the METS. However, genotypes CT+TT (C677T) and AT (rs9939609) were more prevalent in women with high triglyceride levels. Pooled sub-analysis (n = 100) found that median sCD40L and visfatin levels were higher in women with genotypes AT+TT (rs9939609) as compared to AA (1178 vs. 937.0 pg/mL and 0.93 vs. 0.43 ng/mL, respectively, p |
Databáze: | OpenAIRE |
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