Metabolomic changes induced by nicotine in adult zebrafish skeletal muscle
| Autor: | Demetrio Raldúa, Cristian Gómez-Canela, Benjamí Piña, Romà Tauler, Silvia Lacorte, Eva Prats |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Muscle tissue Male medicine.medical_specialty Nicotine Serotonin Health Toxicology and Mutagenesis Metabolite 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Internal medicine Carnitine medicine Toxicity Tests Acute Animals Muscle Skeletal Zebrafish Acetylcholine receptor biology Dose-Response Relationship Drug Chemistry Public Health Environmental and Occupational Health Skeletal muscle General Medicine biology.organism_classification Pollution 030104 developmental biology Endocrinology Nicotinic agonist medicine.anatomical_structure Models Animal Calcium Acetylcarnitine 030217 neurology & neurosurgery medicine.drug Chromatography Liquid |
| Zdroj: | Ecotoxicology and environmental safety. 164 |
| ISSN: | 1090-2414 |
| Popis: | Acute exposure to nicotinic agonists induces myotoxicity in zebrafish embryos. The main goal of this work was to evaluate the potential myotoxicity of nicotine acetylcholine receptor agonists on adult zebrafish muscle tissue by using nicotine as a model compound. Liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) datasets were processed with different chemometric tools based on the selection of Regions of Interest (ROI) and Multivariate Curve-Resolution (ROI-MCR procedure) Alternating Least Squares (ALS) for the analysis of different exposure experiments. Analysis of Variance Simultaneous Component Analysis (ASCA) of changes on metabolite peak profile areas showed significant nicotine concentration and exposure time-dependent changes, clearly differentiating between exposed and non-exposed samples and between short (2 h) and long exposure times (6 h or 24 h). Most of the changes observed in the concentrations of different metabolites are probably secondary to the observed hyperlocomotion, as they have been also observed in humans after strenuous muscular exercise. The absence of myotoxicity might be related with the reduced calcium permeability of adult muscle-type nicotinic acetylcholine receptors (nAChRs). |
| Databáze: | OpenAIRE |
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