Differential expression of long non-coding RNAs in patients with tuberculosis infection

Autor: Shi Lei, Jacky Fong-Chuen Loo, Zhao Chunzhong, Dayong Gu, Boan Li, Qingye Ou, Liu Chunxiao, Siu Kai Kong, Jianan He, Xu Yunqing
Rok vydání: 2017
Předmět:
0301 basic medicine
Microbiology (medical)
Oncology
Genetic Markers
medicine.medical_specialty
Diagnostic methods
Tuberculosis
Microarray
Immunology
Biology
Bioinformatics
Real-Time Polymerase Chain Reaction
Microbiology
Sputum culture
Workflow
Diagnosis
Differential
03 medical and health sciences
0302 clinical medicine
Community-acquired pneumonia
Predictive Value of Tests
Internal medicine
medicine
Humans
In patient
Differential expression
Tuberculosis
Pulmonary
Oligonucleotide Array Sequence Analysis
medicine.diagnostic_test
Gene Expression Profiling
Sputum
Reproducibility of Results
Mycobacterium tuberculosis
medicine.disease
030104 developmental biology
Infectious Diseases
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Case-Control Studies
Host-Pathogen Interactions
RNA
Long Noncoding
Transcriptome
Zdroj: Tuberculosis (Edinburgh, Scotland). 107
ISSN: 1873-281X
Popis: Tuberculosis (TB) remains a major worldwide health problem and has caused millions of deaths in the past few years. Current diagnostic methods, such as sputum smear microscopy and sputum culture, are time-consuming and cannot prevent the rapid spreading of TB during the diagnostic period. In this connection, detecting biomarkers specific to TB at molecular level in plasma of patients will provide a rapid means for diagnosis. In this study, we first evaluated the differential expression of the long non-coding RNAs (lncRNAs) in the plasma from patients with TB (TB positive), community acquired pneumonia (CAP) and healthy individuals (CG) using lncRNA microarray scanning. It was found that there were 2116 specific lncRNAs differentially expressed in the TB positive samples (1102 up-regulated and 1014 down-regulated), which accounted for 6.96% of total lncRNAs. Twelve differentially expressed lncRNAs discovered in microarray were subsequently validated by using real-time quantitative PCR (RT-qPCR). Two lncRNAs (ENST00000354432 and ENST00000427151) were further validated with more Tuberculosis samples. These results suggested the expression level of lncRNAs and the two validated lncRNAs in plasma could be the potential molecular biomarkers for the rapid diagnosis of Tuberculosis.
Databáze: OpenAIRE
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