Molecular Mechanism of Yisui Shengxue Granule, a Complex Chinese Medicine, on Thalassemia Patients Suffering from Hemolysis and Anemia of Erythrocytes

Autor: Wen-juan Wang, Yan-Ling Cheng, Na-Li Chu, Xin-Hua Zhang, Su-ping Fang, Zhi-Kui Wu
Rok vydání: 2014
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine, Vol 2014 (2014)
Evidence-based Complementary and Alternative Medicine : eCAM
ISSN: 1741-4288
1741-427X
DOI: 10.1155/2014/213782
Popis: The objective of this study was to investigate the therapeutic biological mechanism of Yisui Shengxue Granule (YSSXG), a complex Chinese medicine, on the hemolysis and anemia of erythrocytes from patient with thalassemia disease. Sixteen patients with thalassemia (8 cases ofα-thalassemia and 8 cases ofβ-thalassemia) disease were collected and treated with YSSXG for 3 months. The improvements of blood parameter demonstrated that YSSXG had a positive clinical effect on patients with thalassemia disease. For patients withα-thalassemia disease, RT-PCR showed that YSSXG upregulated the relative mRNA expression level ofα-globin toβ-globin and downregulated DNMT1, DNMT3a, and DNMT3b mRNA compared with pretreatment. Western blotting showed that YSSXG downregulated the expression of DNMT1 and DNMT3a. For patients withβ-thalassemia disease, the relative expression level ofAγ-globin toα-globin had an increasing trend and the level of BCL11A mRNA expression obviously increased. For all patients, RT-PCR showed that YSSXG upregulated mRNA expression of SPTA1 and SPTB. Activities of SOD and GSH-Px significantly increased and MDA obviously reduced on erythrocyte and blood serum after YSSXG treatment. TEM showed that YSSXG decreased the content of inclusion bodies. Activities of Na+K+-ATPtase and T-ATPtase of erythrocyte increased significantly after YSSXG treatment. This study provides the basis for mechanisms of YSSXG on thalassemia suffering with hemolysis and anemia of erythrocytes from patient.
Databáze: OpenAIRE
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