Molecular identification and genetic variation studies in economically important cephalopods at Beypore Fishing Harbour (Kozhikode), South West coast of India
| Autor: | Muthusamy Thangaraj, J. Jean Jose, Alex Lincy, Madhavankonath K. Anil |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fishing
Medicine (miscellaneous) Forestry Plant Science Horticulture Agricultural and Biological Sciences (miscellaneous) Biochemistry Genetics and Molecular Biology (miscellaneous) lcsh:S1-972 Fishery Geography Harbour Genetic variation cephalopods cytochrome c oxidase 1 DNA barcoding phylogenetic analysis West coast lcsh:Agriculture (General) lcsh:Science (General) Agronomy and Crop Science computer computer.programming_language Molecular identification lcsh:Q1-390 |
| Zdroj: | Notulae Scientia Biologicae, Vol 13, Iss 1 (2021) |
| ISSN: | 2067-3264 2067-3205 |
| Popis: | Cephalopods are ecologically and economically important marine groups in the world. Biodiversity description is essential for sustainable utilization of natural resources and to characterize biological entities for conservation. DNA barcoding is an effective tool used for identification of organisms at species level and is been widely used for delineate several ambiguity species. In this study, partial sequence of mitochondrial cytochrome c oxidase 1 (CO1) gene with a mean size of 680 bp was amplified by universal primers. Totally 13 individuals of Cephalopods comprising of three species, were barcoded and genetic variation was analysed. The maximum A+T content (67.60%) was recorded in Cistopus indicus and minimum (63.70%) in Sepioteuthis lessoniana. The maximum K2P distance (0.268) was found between the genus Cistopus and Sepioteuthis whereas the minimum distance (0.188) was observed between Uroteuthis and Sepia. The neighbour joining tree revealed three distinct clades represents Loligonidae, Sepiidae and Octopodidae with high boot strap values. However, Sepioteuthis lessoniana is showing a bifurcated branch and it may due to the co-occurring of cryptic species and till date this species is treated as Sepioteuthis lessoniana complex. |
| Databáze: | OpenAIRE |
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