Fate and persistence of antibiotic-resistant bacteria and genes through a multi-barrier treatment facility for direct potable reuse
| Autor: | Katarzyna Slipko, Jörg Krampe, Josef Lahnsteiner, Markus Wögerbauer, Elena Radu, Lina Wallmann, Norbert Kreuzinger, Pierre van Rensburg |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
antibiotic resistance
0208 environmental biotechnology arg Filtration and Separation 02 engineering and technology 010501 environmental sciences Reuse Biology 01 natural sciences arb Environmental technology. Sanitary engineering 020801 environmental engineering Microbiology Persistence (computer science) direct potable reuse Antibiotic resistance Gene Barrier treatment TD1-1066 0105 earth and related environmental sciences Water Science and Technology |
| Zdroj: | Water Reuse, Vol 11, Iss 3, Pp 373-390 (2021) |
| ISSN: | 2709-6106 2709-6092 |
| Popis: | Given the availability of technological solutions and guidelines for safe drinking water, direct potable reuse of reclaimed water has become a promising option to overcome severe lack of potable water in arid regions. However, the growing awareness of the presence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARG) in corresponding raw wastes has led to new safety concerns. This study investigated the fate of ARB and intracellular and extracellular ARG after each treatment step of an advanced water treatment facility in Windhoek, Namibia. The New Goreangab Water Reclamation Plant (NGWRP) produces drinking water from domestic secondary wastewater treatment plant effluent and directly provides for roughly a quarter of Windhoek's potable water demand. Procedures to study resistance determinants were based on both molecular biology and culture-based microbiological methods. TaqMan real-time PCR was employed to detect and quantify intracellular resistance genes sul1, ermB, vanA, nptII and nptIII as well as extracellular resistance gene sul1. The NGWRP reduced the amount of both culturable bacterial indicators as well as the resistance genes to levels below the limit of detection in the final product. The main ozonation and the ultrafiltration had the highest removal efficiencies on both resistance determinants. HIGHLIGHTS The final product contained none of the antibiotic resistance genes investigated.; A subsequent series of water treatment steps can decrease antibiotic resistance genes to below LOD.; All steps in the treatment train decreased the abundance of the sul1 resistance gene except for the pre-ozonation and the biological activated carbon.; In the final product, extracellular and intracellular sul1 resistance genes were below LOD. |
| Databáze: | OpenAIRE |
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