Augmentation of tendon attachment to porous ceramics by bone marrow stromal cells in a rabbit model
| Autor: | Nobuo Adachi, Shin Yokoya, Yu Mochizuki, Mitsuo Ochi, Hiromichi Omae |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
musculoskeletal diseases
Ceramics medicine.medical_specialty Bone Regeneration Stromal cell Knee Joint education Biocompatible Materials Bone Marrow Cells Tendons Implants Experimental parasitic diseases medicine Animals Orthopedics and Sports Medicine Bone regeneration Original Paper business.industry Mesenchymal stem cell Mesenchymal Stem Cells Anatomy musculoskeletal system Biomechanical Phenomena Tendon Apposition Durapatite medicine.anatomical_structure Bone Substitutes Orthopedic surgery Surgery Rabbits Bone marrow business Porosity Biomedical engineering |
| Zdroj: | International Orthopaedics. 31:353-358 |
| ISSN: | 1432-5195 0341-2695 |
| DOI: | 10.1007/s00264-006-0194-8 |
| Popis: | Tendon attachment to interconnected porous calcium hydroxyapatite ceramics (IP-CHA) with cultured bone marrow stromal cells (BMSC) was analysed. The purpose of this study was to evaluate whether BMSC in IP-CHA could augment the tendon attachment to IP-CHA histologically and biomechanically. Eighteen Japanese white rabbits were used. Cultured BMSCs were subcultured in IP-CHA. The grafted tendon and IP-CHA with BMSC complex were implanted in a bone defect of the knee [BMSC(+) group]. In the contralateral knee, a tendon and IP-CHA without BMSC complex were implanted [BMSC(-) group]. Histological findings of the interface between the tendon and IP-CHA were similar in the two groups 3 weeks after the operation. However, 6 weeks after the operation, more abundant bone formation around the tendon was observed in the BMSC(+) group. The direct apposition of the tendon to bone in pores and collagen fibre continuity between the tendon and fibrous tissue in pores were observed. In biomechanical evaluation, the maximum pull-out load of the tendon from the IP-CHA in the BMSC(+) group was significantly higher than that in the BMSC(-) group 6 weeks after the operation. BMSCs cultured in IP-CHA could augment tendon attachment to IP-CHA. |
| Databáze: | OpenAIRE |
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