Reaction time and replenishment time of SP and CGRP after incision in rat skin
| Autor: | Erica Calcagno Raymundo Da Silva, Bernardo Hochman, Lydia Masako Ferreira, Felipe Contoli Isoldi, Paulo Rogério Quieregatto, Gerson Chadi, Guilherme Abbud Franco Lapin, José Octávio Gonçalves de Freitas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
reaction time
Neurogenic inflammation integumentary system General Immunology and Microbiology sp neurogenic inflammation QH301-705.5 General Neuroscience neuropeptides Substance P Anatomy Calcitonin gene-related peptide Scratching Stimulus (physiology) cgrp General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Nociception chemistry Anesthesia Nociceptive Stimulus Biology (General) General Agricultural and Biological Sciences Free nerve ending temporal distribution |
| Zdroj: | Open Life Sciences, Vol 9, Iss 12, Pp 1140-1146 (2014) |
| ISSN: | 2391-5412 |
| Popis: | Background. The skin neurogenic inflammation is mainly related to Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). There is no data on their availability in the dynamics of skin nerve endings, concerning their release and replenishment after a nociceptive stimulus, so this was investigated. Materials and methods. 25 rats were randomly distributed in 5 groups. The animals of the control group (CG) determined the baseline levels of neuropeptides in the skin. The groups S0 and S30 did not receive any cutaneous stimulus at 30 and 60 minutes, respectively. In the group S1, an “incision stimulus” was made at 30 minutes. In the group S31, a nociceptive stimulus was performed by subdermal scratching at 30 minutes and, at 60 minutes, the “incision stimulus” was carried out in the same location (“nociceptive hyperstimulation”). The skin samples of the other animals were harvested from the back 1 minute after their death. SP, pro-CGRP and CGRP were quantified by Western Blotting. Results. The “incision stimulus” released SP, S1 compared to S0 (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|