Quantification of Human Immunodeficiency Virus Type 1 Proviral DNA by Using TaqMan Technology
| Autor: | Mingzhong Chen, Eric G. Bremer, Johann W. Miller, Yuqi Zhao, Min Yu, Ram Yogev, William Kabat |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Human immunodeficiency virus (HIV) HIV Infections medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Virus chemistry.chemical_compound Proviruses Antiretroviral Therapy Highly Active Virology TaqMan medicine Humans Taq Polymerase Child biology RNA Provirus biology.organism_classification Molecular biology chemistry DNA Viral Lentivirus HIV-1 RNA Viral Viral disease DNA |
| Zdroj: | Journal of Clinical Microbiology. 40:675-678 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children. |
| Databáze: | OpenAIRE |
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