Vitamin E-Labeled Polyethylenimine for in vitro and in vivo Gene Delivery
| Autor: | Xinjing Tang, Jiali Yang, Jinxing Liu, Duanwei Liang, Mengke Feng |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Polymers and Plastics
medicine.medical_treatment Gene Expression Bioengineering macromolecular substances 02 engineering and technology Gene delivery 010402 general chemistry 01 natural sciences Cell Line Green fluorescent protein Biomaterials Mice chemistry.chemical_compound In vivo Gene expression Materials Chemistry medicine Animals Humans Polyethyleneimine Vitamin E Lung Polyethylenimine Chemistry Gene Transfer Techniques technology industry and agriculture DNA Genetic Therapy 021001 nanoscience & nanotechnology Molecular biology In vitro 0104 chemical sciences Liver Cell culture 0210 nano-technology Plasmids |
| Zdroj: | Biomacromolecules. 17:3153-3161 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.6b00776 |
| Popis: | A series of Vitamin E (vitE)-labeled PEIs (PEI-vitEn) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitEn/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell lines. PEI-vitE6 showed the best performance in gfp pDNA delivery and following GFP expression in HEK-293A cells. In addition, in vivo gene delivery in living mice also confirmed that PEI-vitE6 showed low toxicity and efficiently delivered gfp pDNA to the cells of liver and lung tissues for gene expression. |
| Databáze: | OpenAIRE |
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