Enzymatic biotransformation of Rb3 from the leaves of Panax notoginseng to ginsenoside rd by a recombinant β-xylosidase from Thermoascus aurantiacus

Autor:	Hui Zhang, Hongxi Zhu, Xiuyuan Luo, Yuanzhen Deng, Wei Zhang, Shubo Li, Jingjuan Liang, Zongwen Pang
Rok vydání:	2022
Předmět:	Plant Leaves Physiology Panax notoginseng Thermoascus General Medicine Applied Microbiology and Biotechnology Biotransformation Biotechnology
Zdroj:	World journal of microbiologybiotechnology. 39(1)
ISSN:	1573-0972
Popis:	Given the important pharmacological activity of ginsenoside Rd but its low content in plants, the production of Rd by enzymatic transformation is of interest. In this study, a β-xylosidase gene Ta-XylQS from Thermoascus aurantiacus was cloned and overexpressed in Komagataella phaffii. Purified recombinant Ta-XylQS specifically hydrolyzes substrates with xylosyl residues at the optimal pH of 3.5 and temperature of 60 °C. This study established a process for producing Rd by transforming ginsenoside Rb3 in the saponins of Panax notoginseng leaves via recombinant Ta-XylQS. After 60 h, 3 g L
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53a85c1842f396a6df55a2739b77ad73 https://pubmed.ncbi.nlm.nih.gov/36422714 Zobrazit plný text záznamu Full text from SpringerLink