Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo
| Autor: | Yoon Hee Jung, Alyx Buonanotte, M. Jordan Rowley, Brianna J. Bixler, Xiaowen Lyu, Anthony W.S. Chan, Victor G. Corces, Kanchana Punyawai, Hannah B. Gold, Isaac Kremsky |
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| Rok vydání: | 2018 |
| Předmět: |
Male
CCCTC-Binding Factor Zygote Embryonic Development RNA polymerase II Biology Article 03 medical and health sciences Mice 0302 clinical medicine Sequence Homology Nucleic Acid Animals Estrogen Receptor beta Humans Enhancer Molecular Biology Conserved Sequence 030304 developmental biology 0303 health sciences Cohesin Base Sequence Estrogen Receptor alpha Gene Expression Regulation Developmental Promoter Embryo Zinc Fingers Cell Biology Embryo Mammalian Sperm Macaca mulatta Spermatozoa Chromatin Cell biology Enhancer Elements Genetic CTCF Receptors Androgen biology.protein Hepatocyte Nuclear Factor 3-beta Oocytes Female 030217 neurology & neurosurgery |
| Zdroj: | Mol Cell |
| ISSN: | 1097-4164 |
| Popis: | The epigenetic information present in mammalian gametes and whether it is transmitted to the progeny are relatively unknown. We find that many promoters in mouse sperm are occupied by RNA polymerase II (Pol II) and Mediator. The same promoters are accessible in GV and MII oocytes and preimplantation embryos. Sperm distal ATAC-seq sites containing motifs for various transcription factors are conserved in monkeys and humans. ChIP-seq analyses confirm that Foxa1, ERα, and AR occupy distal enhancers in sperm. Accessible sperm enhancers containing H3.3 and H2A.Z are also accessible in oocytes and preimplantation embryos. Furthermore, their interactions with promoters in the gametes persist during early development. Sperm- or oocyte-specific interactions mediated by CTCF and cohesin are only present in the paternal or maternal chromosomes, respectively, in the zygote and 2-cell stages. These interactions converge in both chromosomes by the 8-cell stage. Thus, mammalian gametes contain complex patterns of 3D interactions that can be transmitted to the zygote after fertilization. |
| Databáze: | OpenAIRE |
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