Characterization of the immune response to Mycoplasma bovis lung infection
| Autor: | Tony J. Vanden Bush, Ricardo F. Rosenbusch |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Innate immune system
General Veterinary medicine.diagnostic_test Immunology Mycoplasma Biology Lymphocyte Activation medicine.disease_cause Acquired immune system Antibodies Bacterial Lymphocyte Subsets Microbiology Flow cytometry Immune system Antigen medicine Animals Cytokines Cattle Mycoplasma Infections IL-2 receptor Lung CD8 |
| Zdroj: | Veterinary Immunology and Immunopathology. 94:23-33 |
| ISSN: | 0165-2427 |
| Popis: | To better understand the interaction between Mycoplasma bovis and its bovine host, we have characterized the immune response generated during an experimental lung infection with M. bovis. Proliferation ([3H]-thymidine blastogenesis) and Th1/Th2 cytokine production were used to monitor peripheral cellular immune responses. Flow cytometry analysis was used to determine T-cell subset activity by CD25 expression. Humoral immune response was monitored by the identification of antigen-specific IgG1 and IgG2 isotypes over time. Herein, we show that M. bovis antigen stimulates activation of CD4+ and CD8+ cells in vitro in a manner consistent with memory, and that gammadelta-T cells are activated by antigen in a manner consistent with innate immunity. In addition, the percentage of cells producing IFN-gamma during recall response is equal to that of IL-4 producing cells. Serological analysis shows M. bovis stimulates increased production of antigen-specific IgG1 while very little IgG2 is produced. We therefore submit that experimental lung infection of cattle with M. bovis results in a Th2-skewed immune response. |
| Databáze: | OpenAIRE |
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