Different G6PDH isoforms show specific roles in acclimation to cold stress at various growth stages of barley (Hordeum vulgare) and Arabidopsis thaliana
| Autor: | Giorgia Capasso, Simone Landi, Sergio Esposito |
|---|---|
| Přispěvatelé: | Landi, S., Capasso, G., Esposito, S. |
| Rok vydání: | 2021 |
| Předmět: |
Physiology
Acclimatization Arabidopsis Plant Science Glucosephosphate Dehydrogenase Pentose phosphate pathway medicine.disease_cause Redox homeostasi chemistry.chemical_compound Barley Genetics Cold acclimation medicine Protein Isoforms Arabidopsis thaliana 6-Phosphogluconate dehydrogenase (6PGDH) OPPP biology Chemistry Cold-Shock Response Protein Isoform food and beverages ROS Hordeum APX biology.organism_classification Malondialdehyde Biochemistry Cold stre Hordeum vulgare Arabidopsi Oxidative stress |
| Zdroj: | Plant Physiology and Biochemistry. 169:190-202 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2021.11.017 |
| Popis: | Low temperatures (0–10 °C) represent a major physiological stress for plants, negatively affecting both their growth rates and overall growth. Cold stress may induce a wide range of negative physiological effects, from oxidative stress to photosynthetic damage. We investigated the effects of low temperatures in two different model plants, Arabidopsis thaliana and Hordeum vulgare. We tested whether the oxidative pentose phosphate pathway (OPPP) is involved in the increase of reductants’ levels needed to counteract oxidative stress induced by cold. The expression, occurrence, and activity of different glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) isoforms during cold stress and plant recovery from low temperatures, were measured at different growth stages from early germinated to mature pot-grown plants. Our results showed plants exhibited changes in different stress markers; ascorbate peroxidase – APX, catalase - CAT, proline, malondialdehyde, H2O2, NADPH/NADP+. We found that a major role in cold acclimation for cytosolic- and peroxisome-located G6PDHs, and different roles for plastidial/chloroplastic isoforms. This suggests that G6PDH isoforms may regulate redox homeostasis in low temperatures, in order to support the increased and continued demand of reductants during both cold stress and recovery stages. Furthermore, we found a significant involvement of (6PGDH), strengthening the idea that the contribution of the whole oxidative pentose phosphate pathway (OPPP) is required to sustain reductant supply under cold stress. |
| Databáze: | OpenAIRE |
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