Efficient delivery of docetaxel for the treatment of brain tumors by cyclic RGD-tagged polymeric micelles
| Autor: | Wei‑Sheng Liu, Pei‑Cheng Cao, Guo‑Dong Liu, Zhen‑Fu Bu, Ai‑Jun Li, Yue‑Hua Zheng |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Cell Survival Polymers media_common.quotation_subject Brain tumor Antineoplastic Agents Docetaxel Blood–brain barrier Biochemistry Peptides Cyclic Polyethylene Glycols Mice Drug Delivery Systems In vivo Glioma Cell Line Tumor Spheroids Cellular Genetics medicine Tumor Cells Cultured Animals Humans Particle Size Internalization Molecular Biology Micelles media_common Drug Carriers business.industry Brain Neoplasms Cell Cycle medicine.disease Xenograft Model Antitumor Assays Tumor Burden Disease Models Animal Drug Liberation medicine.anatomical_structure Oncology Drug delivery Cancer research Molecular Medicine Taxoids business Drug carrier Glioblastoma medicine.drug |
| Zdroj: | Molecular medicine reports. 11(4) |
| ISSN: | 1791-3004 |
| Popis: | The treatment of glioblastoma, and other types of brain cancer, is limited due to the poor transport of drugs across the blood brain barrier and poor penetration of the blood‑brain‑tumor barrier. In the present study, cyclic Arginine‑Glycine‑Aspartic acid‑D‑Tyrosine‑Lysine [c(RGDyK)], that has a high binding affinity to integrin αvβ3 receptors, that are overexpressed in glioblastoma cancers, was employed as a novel approach to target cancer by delivering therapeutic molecules intracellularly. The c(RGDyK)/docetaxel polylactic acid‑polyethylene glycol (DTX‑PLA‑PEG) micelle was prepared and characterized for various in vitro and in vivo parameters. The specific binding affinity of the Arginine‑Glycine‑Aspartic acid (RGD) micelles, to the integrin receptor, enhanced the intracellular accumulation of DTX, and markedly increased its cytotoxic efficacy. The effect of microtubule stabilization was evident in the inhibition of glioma spheroid volume. Upon intravenous administration, c(RGDyK)/DTX‑PLA‑PEG showed enhanced accumulation in brain tumor tissues through active internalization, whereas non‑targeted micelles showed limited transport ability. Furthermore, RGD‑linked micelles showed marked anti‑glioma activity in U87MG malignant glioma tumor xenografts, and significantly suppressed the growth of tumors without signs of systemic toxicity. In conclusion, the results of the present study suggest that ligand‑mediated drug delivery may improve the efficacy of brain cancer chemotherapy. |
| Databáze: | OpenAIRE |
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