Epidemiology of HHV-8 infection in HIV-positive patients with and without Kaposi sarcoma: diagnostic relevance of serology and PCR
| Autor: | Andreas Plettenberg, Dirk Albrecht, Thore Lorenzen, Ruediger Arndt, Albrecht Stoehr, Thomas Meyer |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty viruses Biology medicine.disease_cause Polymerase Chain Reaction Gastroenterology Herpesviridae law.invention Serology law Germany Virology Internal medicine Immunopathology HIV Seropositivity medicine Humans Gammaherpesvirinae Serologic Tests Sida Sarcoma Kaposi Kaposi's sarcoma Polymerase chain reaction DNA Primers Base Sequence Reproducibility of Results virus diseases Herpesviridae Infections medicine.disease biology.organism_classification Infectious Diseases DNA Viral Herpesvirus 8 Human Immunology Female Viral disease |
| Zdroj: | Journal of Clinical Virology. 30:145-149 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2003.09.017 |
| Popis: | Background: Kaposi’s sarcoma (KS) remains the most common neoplasm in HIV-infected patients. Human herpesvirus 8 (HHV-8) infection is etiologically associated with KS. Diagnostic procedures with regard to HHV-8 infection are not routinely performed in HIV-infected patients; diagnostic and prognostic value of HHV-8 serology or PCR are unknown in this setting. Epidemiological data concerning HHV-8 infection of HIV-infected patients in Germany are rare. Objectives: To assess prevalence of HHV-8 infection in a cohort of HIV-infected patients with and without KS in Germany and to correlate this to manifestations and clinical course of KS. Study design: HHV-8 serology was performed in 483 patients in routine care for HIV-infection in northern Germany. HHV-8 DNA was analyzed by PCR in peripheral blood mononuclear cells (PBMC) of 293 patients; in a subgroup multiple samples were analyzed. History and manifestations of KS were recorded. Results: HHV-8 antibodies were detected using IFT in 91% of 33 patients with KS and 52% of 398 patients without KS. In 36 of 293 (12.3%) patients HHV-8 DNA was detected in PBMC. In general, HHV-8 DNA was not continuously detected when multiple samples from the same patient were analyzed. Patients with KS history were more likely to be PCR positive than those without (45.5% versus 7.8%). In patients with active KS HHV-8 DNA was detected more frequently than in patients with disease remission. HHV-8 DNA was not detected in serologically negative patients. However, three patients with KS history in full remission for several years were seronegative. Conclusions: HIV-infected patients were frequently found to be positive for HHV-8 antibodies. The number of patients positive for viral DNA in PBMC was much smaller. Single PCR-examinations were of little value for prognosis, but repeated detection of HHV-8 DNA represents an increased risk of disease activity. |
| Databáze: | OpenAIRE |
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