Inhalation of hydrogen gas attenuates left ventricular remodeling induced by intermittent hypoxia in mice

Autor: Kenichi Hasegawa, Tatsuhiko Mori, Toshitaka Yoshioka, Yasuo Matsumura, Tetsuya Hayashi, Masatoshi Miyamura, Nobukazu Ishizaka, Akira Ukimura
Rok vydání: 2011
Předmět:
Male
Time Factors
Physiology
Cholesterol
VLDL
medicine.disease_cause
Mice
chemistry.chemical_compound
Superoxides
Fibrosis
Natriuretic Peptide
Brain
Hypoxia
Ventricular Remodeling
Inhalation
Superoxide
Sleep apnea
Intermittent hypoxia
Free Radical Scavengers
Anesthesia
Cardiology
Gases
medicine.symptom
Cardiology and Cardiovascular Medicine
medicine.medical_specialty
Heart Diseases
Heart Ventricles
Physiology (medical)
Internal medicine
Administration
Inhalation
medicine
Animals
RNA
Messenger
Ventricular remodeling
Dyslipidemias
Aldehydes
Analysis of Variance
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
Myocardium
Hemodynamics
Cholesterol
LDL
Hypoxia (medical)
Lipid Metabolism
medicine.disease
Mice
Inbred C57BL
Disease Models
Animal
Oxidative Stress
Gene Expression Regulation
chemistry
business
Oxidative stress
Hydrogen
Zdroj: American Journal of Physiology-Heart and Circulatory Physiology. 301:H1062-H1069
ISSN: 1522-1539
0363-6135
Popis: Sleep apnea syndrome increases the risk of cardiovascular morbidity and mortality. We previously reported that intermittent hypoxia increases superoxide production in a manner dependent on nicotinamide adenine dinucleotide phosphate and accelerates adverse left ventricular (LV) remodeling. Recent studies have suggested that hydrogen (H2) may have an antioxidant effect by reducing hydroxyl radicals. In this study, we investigated the effects of H2gas inhalation on lipid metabolism and LV remodeling induced by intermittent hypoxia in mice. Male C57BL/6J mice ( n = 62) were exposed to intermittent hypoxia (repetitive cycle of 1-min periods of 5 and 21% oxygen for 8 h during daytime) for 7 days. H2gas (1.3 vol/100 vol) was given either at the time of reoxygenation, during hypoxic conditions, or throughout the experimental period. Mice kept under normoxic conditions served as controls ( n = 13). Intermittent hypoxia significantly increased plasma levels of low- and very low-density cholesterol and the amount of 4-hydroxy-2-nonenal-modified protein adducts in the LV myocardium. It also upregulated mRNA expression of tissue necrosis factor-α, interleukin-6, and brain natriuretic peptide, increased production of superoxide, and induced cardiomyocyte hypertrophy, nuclear deformity, mitochondrial degeneration, and interstitial fibrosis. H2gas inhalation significantly suppressed these changes induced by intermittent hypoxia. In particular, H2gas inhaled at the timing of reoxygenation or throughout the experiment was effective in preventing dyslipidemia and suppressing superoxide production in the LV myocardium. These results suggest that inhalation of H2gas was effective for reducing oxidative stress and preventing LV remodeling induced by intermittent hypoxia relevant to sleep apnea.
Databáze: OpenAIRE
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