Inhalation of hydrogen gas attenuates left ventricular remodeling induced by intermittent hypoxia in mice
Autor: | Kenichi Hasegawa, Tatsuhiko Mori, Toshitaka Yoshioka, Yasuo Matsumura, Tetsuya Hayashi, Masatoshi Miyamura, Nobukazu Ishizaka, Akira Ukimura |
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Rok vydání: | 2011 |
Předmět: |
Male
Time Factors Physiology Cholesterol VLDL medicine.disease_cause Mice chemistry.chemical_compound Superoxides Fibrosis Natriuretic Peptide Brain Hypoxia Ventricular Remodeling Inhalation Superoxide Sleep apnea Intermittent hypoxia Free Radical Scavengers Anesthesia Cardiology Gases medicine.symptom Cardiology and Cardiovascular Medicine medicine.medical_specialty Heart Diseases Heart Ventricles Physiology (medical) Internal medicine Administration Inhalation medicine Animals RNA Messenger Ventricular remodeling Dyslipidemias Aldehydes Analysis of Variance Interleukin-6 Tumor Necrosis Factor-alpha business.industry Myocardium Hemodynamics Cholesterol LDL Hypoxia (medical) Lipid Metabolism medicine.disease Mice Inbred C57BL Disease Models Animal Oxidative Stress Gene Expression Regulation chemistry business Oxidative stress Hydrogen |
Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 301:H1062-H1069 |
ISSN: | 1522-1539 0363-6135 |
Popis: | Sleep apnea syndrome increases the risk of cardiovascular morbidity and mortality. We previously reported that intermittent hypoxia increases superoxide production in a manner dependent on nicotinamide adenine dinucleotide phosphate and accelerates adverse left ventricular (LV) remodeling. Recent studies have suggested that hydrogen (H2) may have an antioxidant effect by reducing hydroxyl radicals. In this study, we investigated the effects of H2gas inhalation on lipid metabolism and LV remodeling induced by intermittent hypoxia in mice. Male C57BL/6J mice ( n = 62) were exposed to intermittent hypoxia (repetitive cycle of 1-min periods of 5 and 21% oxygen for 8 h during daytime) for 7 days. H2gas (1.3 vol/100 vol) was given either at the time of reoxygenation, during hypoxic conditions, or throughout the experimental period. Mice kept under normoxic conditions served as controls ( n = 13). Intermittent hypoxia significantly increased plasma levels of low- and very low-density cholesterol and the amount of 4-hydroxy-2-nonenal-modified protein adducts in the LV myocardium. It also upregulated mRNA expression of tissue necrosis factor-α, interleukin-6, and brain natriuretic peptide, increased production of superoxide, and induced cardiomyocyte hypertrophy, nuclear deformity, mitochondrial degeneration, and interstitial fibrosis. H2gas inhalation significantly suppressed these changes induced by intermittent hypoxia. In particular, H2gas inhaled at the timing of reoxygenation or throughout the experiment was effective in preventing dyslipidemia and suppressing superoxide production in the LV myocardium. These results suggest that inhalation of H2gas was effective for reducing oxidative stress and preventing LV remodeling induced by intermittent hypoxia relevant to sleep apnea. |
Databáze: | OpenAIRE |
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