Prenatal alcohol and cannabis exposure can have opposing and region‐specific effects on parvalbumin interneuron numbers in the hippocampus
| Autor: | Kristen R. Breit, Irene Shkolnikov, Hannah M.O. Reid, Cristina G. Rodriguez, Taylor M. Snowden, Jennifer D. Thomas, Brian R. Christie |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Interneuron Medicine (miscellaneous) Hippocampus Toxicology Article Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Interneurons Pregnancy Internal medicine mental disorders medicine Animals Tetrahydrocannabinol Cannabis 030304 developmental biology Cannabinoid Receptor Agonists 0303 health sciences Ethanol biology organic chemicals musculoskeletal neural and ocular physiology Dentate gyrus biology.organism_classification Teratology Rats Psychiatry and Mental health Parvalbumins Endocrinology medicine.anatomical_structure nervous system chemistry Prenatal Exposure Delayed Effects Dentate Gyrus biology.protein Female 030217 neurology & neurosurgery Parvalbumin medicine.drug |
| Zdroj: | Alcohol Clin Exp Res |
| ISSN: | 1530-0277 0145-6008 |
| DOI: | 10.1111/acer.14708 |
| Popis: | BACKGROUND: We have recently shown that alcohol and cannabis can interact prenatally, and in a recent review paper we further identified Parvalbumin-positive (PV) interneurons in the hippocampus as a potential point of convergence for these teratogens. METHODS: A 2 (EtOH, Air) x 2 (THC, Vehicle) design was used to expose pregnant Sprague-Dawley rats to either EtOH or air, in addition to either THC or the inhalant vehicle solution, from gestational days 5–20. Immunohistochemistry was performed to detect parvalbumin-positive interneurons in one male and one female pup from each litter at post-natal day (PND) 70. RESULTS: Significant between-group, and sub-region specific, effects were found in the dorsal CA1 subfield, as well as the ventral DG. In the dorsal CA1 subfield, there was an increase in the number of PV interneurons in both the EtOH and EtOH + THC groups, but only a possible decrease with THC alone. There were fewer changes in interneuron numbers overall in the DG, however, a sex difference was found, and a decrease in the number of PV interneurons in the THC exposed group was observed in the male ventral DG. There was also an increase in cell layer volume between the EtOH + THC group and control group in the DG, and an increase from the control and THC group to the EtOH group in the CA1 region. CONCLUSIONS: Prenatal alcohol and prenatal THC exposure differentially affect parvalbumin interneuron numbers in the hippocampus, indicating that both individual and combined exposure can impact the balance of excitation and inhibition in a structure critically involved in learning and memory processes. |
| Databáze: | OpenAIRE |
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