Systemic and Cutaneous Mucus Antibody Responses of Channel Catfish Immunized against the Protozoan ParasiteIchthyophthirius multifiliis
| Autor: | Harry W. Dickerson, Joanne L. Maki |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Protozoan Vaccines
Microbiology (medical) Time Factors medicine.medical_treatment Blotting Western Clinical Biochemistry Immunology Intraperitoneal injection Antibodies Protozoan Antibodies and Mediators of Immunity Antigens Protozoan Ciliophora Infections Enzyme-Linked Immunosorbent Assay Microbiology Fish Diseases Antigen medicine Animals Immunology and Allergy Ictaluridae biology Ichthyophthirius multifiliis biology.organism_classification Mucus Ictalurus biology.protein Electrophoresis Polyacrylamide Gel Immunization Antibody Catfish |
| Zdroj: | Clinical and Vaccine Immunology. 10:876-881 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | Fish acquire protective immunity against the ciliated protozoan parasiteIchthyophthirius multifiliisfollowing sublethal infection or inoculation withI. multifiliisimmobilization antigens (i-antigens). In both cases, parasite-immobilizing antibodies have been identified in sera and mucosal secretions. To investigate the kinetics of this immune response, antibody levels were determined by enzyme-linked immunosorbent assay (ELISA) in the sera and cutaneous mucus of channel catfish (Ictalurus punctatus) that were either infected with parasites or given a single injection of purified i-antigen (5.0 μg/fish) in Freund's incomplete adjuvant. At 5 weeks, infected and inoculated fish had a mean serum (1:80 dilution) antibody absorbance (A405) value of 0.54 ± 0.17 and 0.35 ± 0.03, respectively, which were significantly higher (α = 0.05) than the pretreatment serum (1:80 dilution) antibody absorbance value of 0.24 ± 0.05. At 14 weeks, mean serum (1:80 dilution) ELISA absorbance values in the teo groups of fish increased to 0.79 ± 0.30 and 0.71 ± 0.24, respectively. In both groups of fish, antibody levels in cutaneous mucus (undiluted) were much lower than those in sera. Infected fish had detectable mucus (undiluted) antibody levels from 3 to 9 weeks, with the highest mean value (0.30 ± 0.07) occurring at 7 weeks. Although individual inoculated fish produced serum antibody absorbance values comparable to those seen in infected fish, the mean mucus antibody values in this group did not rise above pretreatment levels.I. multifiliisinfection induced a transient mucosal antibody response that coincided with the resolution of infection. Whether elicited by infection or intraperitoneal injection of i-antigen, the serum and mucus antibody responses of channel catfish immunized againstI. multifiliisdid not occur synchronously. |
| Databáze: | OpenAIRE |
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