The outer chloroplast envelope protein OEP16-1 for plastid import of NADPH:protochlorophyllide oxidoreductase A in Arabidopsis thaliana
| Autor: | Abder Lahroussi, Christiane Reinbothe, Steffen Reinbothe, Frank Buhr, Armin Springer, Stephan Pollmann, Iga Samol, Diter von Wettstein, Claudia Rossig |
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| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
Physiology Biología Mutant Arabidopsis Plant Science Biology 01 natural sciences Chloroplast membrane Ion Channels 03 medical and health sciences Protochlorophyllide Oxidoreductase Complementary DNA Plastids Plastid Plastid envelope Gene 030304 developmental biology chemistry.chemical_classification 0303 health sciences Arabidopsis Proteins Genetic Complementation Test Cell Biology General Medicine Cell biology chemistry Biochemistry Mutation 010606 plant biology & botany |
| Zdroj: | Plant And Cell Physiology, ISSN 0032-0781, 2011-01, Vol. 52, No. 1 Archivo Digital UPM instname |
| ISSN: | 1471-9053 |
| Popis: | The outer plastid envelope protein OEP16-1 was previously identified as an amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA). Reverse genetic approaches used to dissect these mutually not exclusive functions of OEP16-1 in planta have led to descriptions of different phenotypes resulting from the presence of several mutant lines in the SALK_024018 seed stock. In addition to the T-DNA insertion in the AtOEP16-1 gene, lines were purified that contain two additional T-DNA insertions and as yet unidentified point mutations. In a first attempt to resolve the genetic basis of four different lines in the SALK_024018 seed stock, we used genetic transformation with the OEP16-1 cDNA and segregation analyses after crossing out presumed point mutations. We show that AtOEP16-1 is involved in PORA precursor import and by virtue of this activity confers photoprotection onto etiolated seedlings during greening. |
| Databáze: | OpenAIRE |
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