Identification and Quantification of Coumarins by UHPLC-MS in Arabidopsis thaliana Natural Populations
| Autor: | Frédéric Bourgaud, Alain Hehn, Anna Ihnatowicz, Joanna Siwinska, Jérémy Grosjean, Alexandre Olry, Izabela Perkowska, Ewa Lojkowska |
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| Přispěvatelé: | University of Gdańsk (UG), Laboratoire Agronomie et Environnement (LAE), Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plant Advanced Technologies S.A. |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
natural products [SDV]Life Sciences [q-bio] Arabidopsis natural genetic variation Pharmaceutical Science Umbelliferone Plant Roots 01 natural sciences Article Mass Spectrometry Analytical Chemistry lcsh:QD241-441 Metabolic engineering 03 medical and health sciences chemistry.chemical_compound model plant lcsh:Organic chemistry Coumarins Scopoletin Drug Discovery Arabidopsis thaliana heterocyclic compounds Physical and Theoretical Chemistry Chromatography High Pressure Liquid 030304 developmental biology chemistry.chemical_classification 0303 health sciences simple coumarins biology Organic Chemistry Glycoside Coumarin biology.organism_classification analytical methods Biochemistry chemistry Chemistry (miscellaneous) Molecular Medicine Scopolin 010606 plant biology & botany |
| Zdroj: | Molecules Molecules, MDPI, 2021, 26 (6), pp.1804. ⟨10.3390/molecules26061804⟩ Molecules, Vol 26, Iss 1804, p 1804 (2021) Volume 26 Issue 6 |
| ISSN: | 1420-3049 |
| Popis: | International audience; Coumarins are phytochemicals occurring in the plant kingdom, which biosynthesis is induced under various stress factors. They belong to the wide class of specialized metabolites well known for their beneficial properties. Due to their high and wide biological activities, coumarins are important not only for the survival of plants in changing environmental conditions, but are of great importance in the pharmaceutical industry and are an active source for drug development. The identification of coumarins from natural sources has been reported for different plant species including a model plant Arabidopsis thaliana. In our previous work, we demonstrated a presence of naturally occurring intraspecies variation in the concentrations of scopoletin and its glycoside, scopolin, the major coumarins accumulating in Arabidopsis roots. Here, we expanded this work by examining a larger group of 28 Arabidopsis natural populations (called accessions) and by extracting and analysing coumarins from two different types of tissues–roots and leaves. In the current work, by quantifying the coumarin content in plant extracts with ultra-high-performance liquid chromatography coupled with a mass spectrometry analysis (UHPLC-MS), we detected a significant natural variation in the content of simple coumarins like scopoletin, umbelliferone and esculetin together with their glycosides: scopolin, skimmin and esculin, respectively. Increasing our knowledge of coumarin accumulation in Arabidopsis natural populations, might be beneficial for the future discovery of physiological mechanisms of action of various alleles involved in their biosynthesis. A better understanding of biosynthetic pathways of biologically active compounds is the prerequisite step in undertaking a metabolic engineering research. |
| Databáze: | OpenAIRE |
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