Platelet reticulated forms, size indexes and functional activity. Interactions in healthy volunteers
| Autor: | Valeria V Bodrova, Olga N Shustova, Svetalana G Khaspekova, Mazurov Av |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Blood Platelets medicine.medical_specialty Adolescent Platelet Function Tests Population 030204 cardiovascular system & hematology Flow cytometry 03 medical and health sciences Young Adult 0302 clinical medicine Internal medicine Healthy volunteers medicine Humans Platelet Platelet activation Mean platelet volume education education.field_of_study medicine.diagnostic_test Chemistry Platelet Count Hematology General Medicine Middle Aged Healthy Volunteers Staining 030104 developmental biology medicine.anatomical_structure Endocrinology Case-Control Studies Bone marrow |
| Zdroj: | Platelets. 33(3) |
| ISSN: | 1369-1635 |
| Popis: | Reticulated platelets (RP) are young, functionally active platelet forms which are detected by RNA staining. Their content in the circulation reflects the intensity of bone marrow thrombocytopoesis. The aim of this study was to assess in healthy volunteers the relationship between RP percentage and platelet size and activity. RP were quantitated by thiazole orange staining using flow cytometry. Platelet size indexes included mean platelet volume (MPV), platelet large cell ratio (P-LCR) measured in a Coulter type hematological analyzer and forward scattering (FSC) measured in a flow cytometer. Platelet functional activity was evaluated by expression of activated glycoprotein (GP) IIb-IIIa (PAC-1 antibody binding) and P-selectin with the use of flow cytometry. Platelets were activated by thrombin receptor activating peptide (TRAP) (10 and 1 µM) and ADP (20 and 2.5 µM). The percentage of RP in healthy volunteers varied from 2.9% to 23.8% (mean ± SD ‒ 11.7 ± 4.7%, n = 99) and correlated with all platelet size indexes: MPV, P-LCR and FCS (r from 0.452 to 0.529, p < .001, n = 87-99). On average, RP were distributed at a ratio of 9:1 between 50% subpopulations of large and small platelets according to their FSC index. Expression of GP IIb-IIIa activated form correlated with RP percentage and platelet size indexes when platelets were activated by TRAP and ADP at both applied concentrations (r from 0.309 to 0.560, p from 0.014 to < 0.001, n = 50-62). P-selectin expression correlated with RP percentage and platelet size indexes when platelets were activated by 10 µM TRAP inducing maximum expression of this activation marker (r from 0.332 to 0.556, p from 0.008 to < 0.001, n = 65), but not by weaker agonists: 1 µM TRAP, 20 and 2.5 µM ADP (r < 0.3, n = 54-66). Thus, high RP content in healthy volunteers is associated with increased platelet size and activity in the whole platelet population. |
| Databáze: | OpenAIRE |
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