Interstitial cells of Cajal mediate excitatory sympathetic neurotransmission in guinea pig prostate
| Autor: | Guo-fu Ding, Jiang-ping Wang, Qin-zhang Wang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Sympathetic nervous system Sympathetic Nervous System Histology Tyrosine 3-Monooxygenase Guinea Pigs Myocytes Smooth Muscle Connexin Cell Separation Dopamine beta-Hydroxylase Biology Neurotransmission Synaptic Transmission Pathology and Forensic Medicine Norepinephrine symbols.namesake Prostate Receptors Adrenergic alpha-1 Internal medicine medicine Prazosin Animals Humans Cells Cultured Staining and Labeling Tyrosine hydroxylase Cell Biology Interstitial Cells of Cajal Immunohistochemistry Interstitial cell of Cajal Proto-Oncogene Proteins c-kit medicine.anatomical_structure Endocrinology Adrenergic alpha-1 Receptor Antagonists symbols medicine.drug |
| Zdroj: | Cell and Tissue Research. 352:479-486 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-013-1572-3 |
| Popis: | Morphological and functional studies have confirmed that interstitial cells of Cajal (ICCs) are involved in many enteric motor neurotransmission pathways. Recent investigations have demonstrated that human and guinea pig prostate glands possess a distinct cell type with morphological and immunological similarities to ICCs. These prostate ICCs have a close relationship with nerve bundles and smooth muscle cells. Prostate smooth muscle tone is largely induced by stimulation from the sympathetic nervous system, which releases excitatory norepinephrine (NE) to act on the α1-adrenoceptor. We have performed morphological and functional experiments to determine the role of ICCs in sympathetic neurotransmission in the guinea pig prostate based on the hypothesis that prostate ICCs act as mediators of sympathetic neurotransmission. Immunohistochemistry revealed many close points of contact between ICCs and sympathetic nerve bundles and smooth muscle cells. Double-labeled sections revealed that α1-adrenoceptor and the gap junction protein connexin 43 were expressed in prostate ICCs. Surprisingly, prostate ICCs co-expressed tyrosine hydroxylase and dopamine β-hydroxylase, two markers of sympathetic neurons. Functionally, the application of NE evoked a large single inward current in isolated prostate ICCs in a dose-dependent manner. The inward current evoked by NE was mediated via the activation of α1-adrenoceptors, because it was abolished by the non-specific α-adrenoceptor antagonist, phentolamine and the specific α1-adrenoceptor antagonist, prazosin. Thus, ICCs in the guinea pig prostate are target cells for prostate sympathetic nerves and possess the morphological and functional characteristics required to mediate sympathetic signals. |
| Databáze: | OpenAIRE |
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