Air-side ammonia stripping coupled to anaerobic digestion indirectly impacts anaerobic microbiome
Autor: | Marta Carballa, Juan M. Lema, Chiara Pedizzi, Nuria Fernandez-Gonzalez |
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Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Enxeñaría Química |
Rok vydání: | 2018 |
Předmět: |
Hot Temperature
Firmicutes Swine lcsh:Biotechnology Bioengineering Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences Ammonia chemistry.chemical_compound Bacteria Anaerobic Bioreactors lcsh:TP248.13-248.65 Animals Anaerobiosis Anaerobic microbiome Research Articles 030304 developmental biology 2. Zero hunger 0303 health sciences biology 030306 microbiology Thermophile Microbiota Air-side Hydrogen-Ion Concentration biology.organism_classification Manure Archaea 6. Clean water Anaerobic digestion Methanoculleus chemistry Microbial population biology 13. Climate action Environmental chemistry Biotechnology Research Article |
Zdroj: | Microbial Biotechnology Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Microbial Biotechnology, Vol 12, Iss 6, Pp 1403-1416 (2019) |
ISSN: | 1751-7915 |
Popis: | Air‐side stripping without a prior solid–liquid phase separation step is a feasible and promising process to control ammonia concentration in thermophilic digesters. During the process, part of the anaerobic biomass is exposed to high temperature, high pH and aerobic conditions. However, there are no studies assessing the effects of those harsh conditions on the microbial communities of thermophilic digesters. To fill this knowledge gap, the microbiomes of two thermophilic digesters (55°C), fed with a mixture of pig manure and nitrogen‐rich co‐substrates, were investigated under different organic loading rates (OLR: 1.1–5.2 g COD l−1 day−1), ammonia concentrations (0.2–1.5 g free ammonia nitrogen l−1) and stripping frequencies (3–5 times per week). The bacterial communities were dominated by Firmicutes and Bacteroidetes phyla, while the predominant methanogens were Methanosarcina sp archaea. Increasing co‐substrate fraction, OLR and free ammonia nitrogen (FAN) favoured the presence of genera Ruminiclostridium, Clostridium and Tepidimicrobium and of hydrogenotrophic methanogens, mainly Methanoculleus archaea. The data indicated that the use of air‐side stripping did not adversely affect thermophilic microbial communities, but indirectly modulated them by controlling FAN concentrations in the digester. These results demonstrate the viability at microbial community level of air side‐stream stripping process as an adequate technology for the ammonia control during anaerobic co‐digestion of nitrogen‐rich substrates This research was supported by the European Community Seventh Framework Programme (ManureEcoMine project – 603744); and by the Spanish Government (AEI) through CDTI (SmartGreenGas project – 2014-CE224). The authors belong to the Galician Competitive Research Group ED431C 2017/029 and to the CRETUS Strategic Partnership (ED431E 2018/01), co-funded by FEDER (UE). Computational resources were kindly provided and supported by Fundacion Publica Galega Centro Tecnolóxico de Supercomputación de Galicia (CESGA) SI |
Databáze: | OpenAIRE |
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