Isolation and characterization of mouse monoclonal antibodies reactive with soft tick (Acari: Argasidae) salivary antigens of high molecular weight
| Autor: | Stephen G. Zam, Jerry F. Butler, James T. Need, Edward J. Wozniak |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.drug_class
Tick Monoclonal antibody Salivary Glands Mice Ticks Antigen medicine Animals Antigens Ornithodoros Antiserum Mice Inbred BALB C General Veterinary biology Salivary gland Antibodies Monoclonal biology.organism_classification Virology Molecular biology Molecular Weight Infectious Diseases medicine.anatomical_structure Insect Science Monoclonal biology.protein Parasitology Female Antibody |
| Zdroj: | Journal of medical entomology. 33(2) |
| ISSN: | 0022-2585 |
| Popis: | The purpose of this study was to immunologically characterize soft tick salivary antigens. BALB/c mice hyperimmunized with salivary gland extract prepared from Ornithodoros talaje (Guerin-Meneville) were observed to develop high titers of antitick salivary antigen antibodies. Subsequent fusion of splenic lymphocytes from the hyperimmunized mice with SP-2/0-AG14 myeloma cells resulted in the production of 10 antitick IgM-producing hybridoma clones. Partial characterization of the respective tick antigens by gel filtration and SDS-PAGE demonstrated all 10 monoclonal antitick antibodies to be reactive with a salivary gland extract fraction containing proteins 50-110 kDa in molecular weight. Cross-reactivity assays and electrophoretic comparison of salivary gland extract specimens demonstrated similar proteins in several ixodid tick genera and species. |
| Databáze: | OpenAIRE |
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