Identification of STN7/STN8 kinase targets reveals connections between electron transport, metabolism and gene expression
Autor: | Wolfgang Hoehenwarter, Stefan Helm, Anja Rödiger, Sacha Baginsky, Johann Galonska, Petra Majovsky, Gideon Christ, Anna Schönberg, Domenika Thieme, Wiebke Mehwald |
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Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine Protein subunit Mutant Arabidopsis Light-Harvesting Protein Complexes Plant Science Protein Serine-Threonine Kinases 01 natural sciences Electron Transport 03 medical and health sciences Ribosomal protein Genetics Protein phosphorylation Phosphorylation Photosynthesis biology Arabidopsis Proteins Kinase RuBisCO Phosphoproteomics Photosystem II Protein Complex Cell Biology Cell biology 030104 developmental biology Biochemistry biology.protein Protein Kinases 010606 plant biology & botany |
Zdroj: | The Plant Journal. 90:1176-1186 |
ISSN: | 0960-7412 |
Popis: | Summary The thylakoid-associated kinases STN7 and STN8 are involved in short- and long-term acclimation of photosynthetic electron transport to changing light conditions. Here we report the identification of STN7/STN8 in vivo targets that connect photosynthetic electron transport with metabolism and gene expression. Comparative phosphoproteomics with the stn7 and stn8 single and double mutants identified two proteases, one RNA-binding protein, a ribosomal protein, the large subunit of rubisco and a ferredoxin-NADP reductase as targets for the thylakoid-associated kinases. Phosphorylation of three of the above proteins can be partially complemented by STN8 in the stn7 single mutant albeit at lower efficiency, while phosphorylation of the remaining three proteins strictly depends on STN7. The properties of the STN7-dependent phosphorylation site are similar to those of phosphorylated light-harvesting complex proteins entailing glycine or another small hydrophobic amino acid in the -1 position. Our analysis uncovers the STN7/STN8 kinases as mediators between photosynthetic electron transport, its immediate downstream sinks and long–term adaptation processes affecting metabolite accumulation and gene expression. This article is protected by copyright. All rights reserved. |
Databáze: | OpenAIRE |
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