3D imaging of human epidermis micromorphology by combining fluorescent dye, optical clearing and confocal microscopy

Autor:	Eric Fernandez, Sylvie Marull-Tufeu
Rok vydání:	2019
Předmět:	Materials science Microscope Confocal Dermatology 01 natural sciences law.invention 010309 optics 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound Imaging Three-Dimensional 0302 clinical medicine law Confocal microscopy 0103 physical sciences medicine Fluorescence microscope Rhodamine B Stratum corneum Humans Fluorescent Dyes Microscopy Confocal integumentary system Rhodamines Fluorescence medicine.anatomical_structure Epidermal Cells Microscopy Fluorescence chemistry Biophysics Epidermis
Zdroj:	Skin Research and Technology. 25:735-742
ISSN:	1600-0846 0909-752X
DOI:	10.1111/srt.12710
Popis:	Background The human epidermis presents a complex organization due to its dynamic and plein-stratified epithelium. Still nowadays, one of the best method to study this layer of the skin remains the histology sections. This approach remains tedious and gives only 2D information. Materials and methods Rhodamine B is a dye known to have a high affinity for the epidermal layer and to possess fluorescent properties. Associated with a clarification method such as 3DiSCO, this dye maintains a high fluorescence emission. The skin which became transparent can then be investigated with a Laser Scanning Confocal Microscope. Results We showed that this technique can collect longitudinal or transversal optical sections of the epidermis as whole. Serial sections allowed to move easily into the epidermis. A 3D imaging can also be generated to study the microrelief of the stratum corneum or the complex organization of the dermal-epidermal junction. Conclusion In this work, we describe a simple and fast staining and clearing method for skin samples. In association with a fluorescence microscope such as the confocal, we show a new way to characterize the whole epidermis. This work appears as a valuable and complementary method to understand several topics around skin investigation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::539014ff6efd3557691158289912087f https://doi.org/10.1111/srt.12710 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.