Early delayed radiation-induced brain injury in mice: preliminary findings using magnetic resonance imaging
| Autor: | Anthony J. Chalmers, Rodrigo Gutierrez-Quintana, Apostolos Zarros, Lindsay Gallagher, Abdulrahman Hussain Qaisi, Antoine Vallatos, William M. Holmes, Katrina Stevenson, Lesley Gilmour |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty External capsule medicine.diagnostic_test Glial fibrillary acidic protein biology business.industry medicine.medical_treatment Central nervous system Hippocampus Magnetic resonance imaging Radiation therapy Abstracts medicine.anatomical_structure Oncology medicine Medical imaging biology.protein Neurology (clinical) business Diffusion MRI |
| Popis: | Radiotherapy has improved survival outcomes for central nervous system (CNS) malignancies. However, the negative impact of radiotherapy on the healthy tissue surrounding these malignancies, is increasingly becoming a concern. We aimed to investigate whether the employment of magnetic resonance imaging (MRI) techniques could be used to detect crucial aspects of the time-dependent effects resulting from the exposure of the CNS to ionizing radiation (IR). We hereby report preliminary MRI findings of a study examining the early delayed IR-induced CNS injury in adult CD-1 nude mice that had their right brain hemisphere exposed to a 20 Gy single IR dose by a Small Animal Radiation Research Platform (SARRP). T(2) mapping and multiple b value diffusion weighted imaging (DWI) with a range of observation times of the whole brain were acquired with a 7T small animal preclinical MRI scanner at 6 time-points: before irradiation and then at 1, 10, 17, 60 and 80 days post irradiation. The acquired T(2) mapping data indicated no significant change in actual T(2) values and revealed no significant deviation from normal mono-exponential decay. Similarly, DWI data with short observation times (20–80 ms) showed no significant deviation from Gaussian behaviour, suggesting the existence of no CNS microstructural changes due to IR. However, multiple b value DWI with a 200 ms observation time showed deviations from Gaussian behaviour, suggesting that the assessment of diffusion kurtosis imaging (DKI) could be informative for identifying early delayed radiation-induced brain injury in mice. Our promising MRI findings are examined in parallel with neuropathological observations in the brain tissue of these mice (obtained at 80 days post irradiation), resulting from the assessment of haematoxylin-eosin, cresyl violet, glial fibrillary acidic protein (GFAP) and Luxol fast blue staining in CNS structures of relevance (such as the hippocampus, fimbria, external capsule, thalamus and selected cortical regions). |
| Databáze: | OpenAIRE |
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